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TBL1XR1 as a potential therapeutic target that promotes epithelial-mesenchymal transition in lung squamous cell

Yuehua Zhao1,2, Hao Lin1,2, Jingwei Jiang1,2

  • 1Department of Oncology, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.

Experimental and Therapeutic Medicine
|January 18, 2019
PubMed

Insights

Transducin (β)-like 1 X-linked receptor 1 (TBL1XR1) promotes lung squamous cell carcinoma (SCC) progression by enhancing cell proliferation, invasion, and migration. TBL1XR1 may be a therapeutic target for lung SCC by influencing epithelial-mesenchymal transition via the TGF-β/Smad pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Transducin (β)-like 1 X-linked receptor 1 (TBL1XR1) is implicated in tumor progression, but its role in lung squamous cell carcinoma (SCC) is unclear.
  • Understanding TBL1XR1's function in lung SCC is crucial for identifying new therapeutic strategies.

Purpose of the Study:

  • To investigate the biological role and molecular mechanisms of TBL1XR1 in lung SCC.
  • To determine if TBL1XR1 influences cell proliferation, invasion, migration, and epithelial-mesenchymal transition (EMT) in lung SCC.

Main Methods:

  • TBL1XR1 expression was analyzed in lung SCC cell lines.
  • In vitro assays (cell counting kit-8, invasion, wound healing) assessed proliferation, invasion, and migration.
  • Western blotting examined EMT markers and the transforming growth factor (TGF)-β/mothers against decapentaplegic homolog (Smad) signaling pathway.

Main Results:

  • TBL1XR1 was upregulated in lung SCC cells.
  • Overexpression of TBL1XR1 enhanced proliferation, invasion, and migration, inducing a mesenchymal phenotype.
  • TBL1XR1 modulated EMT markers and the TGF-β/Smad pathway, increasing E-cadherin and decreasing SNAI1, ZEB1, p-Smad2/3, Smad2, and Smad3.

Conclusions:

  • TBL1XR1 plays a significant role in lung SCC development and progression.
  • TBL1XR1 may promote lung SCC by inducing EMT through activation of the TGF-β/Smad signaling pathway.
  • TBL1XR1 represents a potential therapeutic target for lung SCC.
Keywords:
epithelial-mesenchymal transitioninvasionlung squamous cell carcinomasmigrationproliferationtransducin (β)-like 1 X-linked receptor 1transforming growth factor-β/mothers against decapentaplegic homolog signaling pathway

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