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Glucose affects cell viability, migration, angiogenesis and cellular adhesion of human retinal capillary endothelial

Yang Fu1,2, Min Tang1,2, Xiaoqiong Xiang3

  • 1Department of Ophthalmology, Shanghai General Hospital of Nanjing Medical University, Shanghai 200080, P.R. China.

Experimental and Therapeutic Medicine
|January 18, 2019
PubMed

Insights

Secreted protein acidic and rich in cysteine (SPARC) exacerbates diabetic retinopathy by impairing human retinal capillary endothelial cells. Inhibiting SPARC expression partially reversed high glucose-induced damage, suggesting SPARC

Area of Science:

  • Ophthalmology
  • Endocrinology
  • Molecular Biology

Background:

  • Diabetic retinopathy (DR) is a significant complication of diabetes mellitus.
  • Secreted protein acidic and rich in cysteine (SPARC) expression is linked to DR pathology.
  • The precise role of SPARC in retinal endothelial cells under hyperglycemic conditions requires elucidation.

Purpose of the Study:

  • To investigate the regulatory role of SPARC in human retinal capillary endothelial cells (HRCECs).
  • To assess the impact of high glucose on HRCECs and the involvement of SPARC.
  • To evaluate the therapeutic potential of SPARC inhibition in a high glucose environment.

Main Methods:

  • HRCECs were exposed to varying high glucose concentrations (25, 50, 100 mM).
  • SPARC expression was modulated using lentiviral vectors to achieve downregulation.
  • Cell viability (CCK-8 assay), migration, angiogenesis, and tight/adherens/gap junction protein expression were analyzed.

Main Results:

  • High glucose significantly increased SPARC expression in HRCECs.
  • Hyperglycemia inhibited HRCEC proliferation, migration, angiogenesis, and junctional protein expression.
  • Downregulation of SPARC partially reversed these detrimental effects induced by high glucose.

Conclusions:

  • SPARC plays a crucial role in mediating the adverse effects of high glucose on HRCECs.
  • Inhibition of SPARC can ameliorate glucose-induced damage to retinal endothelial cells.
  • SPARC represents a potential therapeutic target for managing diabetic retinopathy.
Keywords:
SPARCdiabetic retinopathyhuman retinal capillary endothelial cellssecreted protein acidic and rich in cysteine

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