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Quantification of Mouse Hematopoietic Progenitors' Formation Using Time-lapse Microscopy and Image Analysis
Isabelle Bergiers1, Christian Tischer2, Özge Vargel Bölükbaşı1
1European Molecular Biology Laboratory, EMBL Rome, Epigenetics and Neurobiology Unit Monterotondo, Italy.
Bio-Protocol
|January 18, 2019
Summary
This study quantifies mouse hematopoietic progenitor formation during endothelial-to-hematopoietic transition (EHT) using time-lapse microscopy. It assesses how transcription factors affect blood cell development in vitro.
Area of Science:
- Developmental Biology
- Stem Cell Biology
- Hematopoiesis
Background:
- * Mouse embryonic stem cells (mESCs) differentiation is a model for studying blood development.
- * The endothelial-to-hematopoietic transition (EHT) is crucial for initiating blood formation.
- * Quantifying hematopoietic progenitor emergence is key to understanding EHT.
Purpose of the Study:
- * To describe a method for quantifying mouse hematopoietic progenitors during EHT.
- * To evaluate EHT progression under normal conditions.
- * To assess the impact of over-expressing eight transcription factors on EHT.
Main Methods:
- * Utilized time-lapse microscopy for live cell imaging.
- * Employed image analysis techniques to quantify cell formation.
- * Differentiated mouse embryonic stem cells (mESCs) in vitro.
Main Results:
- * Established a method to quantify hematopoietic progenitor formation during EHT.
- * Observed changes in progenitor emergence following transcription factor over-expression.
- * Provided a tool to assess factors influencing blood development.
Conclusions:
- * Developed a non-invasive method to monitor EHT and hematopoietic progenitor formation.
- * Demonstrated the utility of time-lapse microscopy and image analysis for studying blood development.
- * Identified transcription factors that modulate the EHT process.