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Related Experiment Video

Updated: Jan 30, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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OsDCL3b affects grain yield and quality in rice.

Peng-Fei Liao1, Jie-Xiu Ouyang1, Jian-Jun Zhang2

  • 1Key Laboratory of Molecular Biology and Gene Engineering in Jiangxi Province, School of Life Sciences, Nanchang University, Nanchang, 330031, China.

Plant Molecular Biology
|January 18, 2019
PubMed
Summary

Knocking down OsDCL3b in rice reduces grain yield but improves grain quality by affecting small RNA biogenesis and gene expression, aiding molecular breeding.

Keywords:
Grain qualityGrain yieldOsDCL3bPollen fertilitySeed-setting rateSmall RNA

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Area of Science:

  • Plant molecular biology
  • Genetics
  • Crop science

Background:

  • Multiple DICER-LIKE (DCL) genes in plants have diverse functions.
  • OsDCL3b in rice processes 24-nucleotide phased small RNAs, but its phenotypic roles are unknown.

Purpose of the Study:

  • To investigate the phenotypic functions of OsDCL3b in rice.
  • To elucidate the molecular mechanisms underlying OsDCL3b's effects on rice yield and quality.

Main Methods:

  • RNA interference (RNAi) to knockdown OsDCL3b expression.
  • Deep sequencing of small RNAs and RNA sequencing (RNA-seq) of rice panicles.
  • Analysis of messenger RNA (mRNA) gene expression and alternative splicing (AS) events.

Main Results:

  • Knockdown of OsDCL3b reduced pollen fertility, seed setting rate, grain yield, but increased grain quality.
  • OsDCL3b knockdown altered the biogenesis of 21- and 24-nucleotide small RNAs, including microRNAs (miRNAs) and phased small RNAs.
  • Differential expression of 644 up- and 530 down-regulated mRNA genes and altered alternative splicing events were observed.

Conclusions:

  • OsDCL3b plays a crucial role in regulating small RNA biogenesis, mRNA transcription, and alternative splicing in rice panicles.
  • Modulating OsDCL3b impacts rice grain yield and quality, offering potential for molecular breeding strategies.