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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Gene Editing in Sorghum Through Agrobacterium.

Jeffry D Sander1

  • 1Corteva Agriscience™, Agriculture Division of DowDuPont™, Johnston, IA, USA. jeffry.sander@pioneer.com.

Methods in Molecular Biology (Clifton, N.J.)
|January 18, 2019
PubMed
Summary

CRISPR/Cas gene editing in sorghum accelerates genetic research and crop improvement. This technology, combined with advanced tissue culture and Agrobacterium methods, enables precise genetic modifications for developing hardier, more productive sorghum varieties.

Keywords:
AgrobacteriumCRISPRCRISPR-CasCRISPR/CasCas9Gene editingSorghum

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Area of Science:

  • Agricultural Science
  • Genetics
  • Biotechnology

Background:

  • CRISPR/Cas technology enables targeted genomic edits, advancing genetic research.
  • Sorghum is a vital staple crop, particularly in food-insecure regions.
  • Understanding sorghum gene function is crucial for crop improvement.

Purpose of the Study:

  • To describe CRISPR/Cas gene editing technology.
  • To outline strategies for applying CRISPR/Cas in sorghum using Agrobacterium.
  • To facilitate the study of gene function and crop development in sorghum.

Main Methods:

  • CRISPR/Cas gene editing.
  • Sorghum tissue culture techniques.
  • Agrobacterium-mediated transformation.

Main Results:

  • CRISPR/Cas facilitates targeted genomic modifications in sorghum.
  • Integration of CRISPR/Cas with existing technologies enhances its applicability.
  • Potential for introducing desirable traits and natural variations into sorghum lines.

Conclusions:

  • CRISPR/Cas gene editing is a powerful tool for sorghum research and development.
  • The combination of CRISPR/Cas, tissue culture, and Agrobacterium offers a robust platform for crop improvement.
  • This approach can accelerate the development of agriculturally relevant sorghum varieties.