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Updated: Jan 30, 2026

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
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Assaying Sorghum for Fuel Production.

Courtney Payne1, Justin Sluiter2, Ed Wolfrum1

  • 1The National Renewable Energy Laboratory, Golden, CO, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 18, 2019
PubMed
Summary

This study details NREL laboratory analytical procedures (LAPs) for characterizing sorghum feedstock composition. These methods analyze key compounds like starch, glucan, and lignin across different sorghum varieties.

Keywords:
CelluloseCharacterizationGlucanHemicelluloseLAPsLigninLignocelluloseNonstructural carbohydratesSorghumStarchStructural carbohydratesXylan

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Area of Science:

  • Biomass and Bioenergy Analysis
  • Plant Biochemistry and Composition

Background:

  • Accurate feedstock characterization is crucial for developing advanced biofuels.
  • The National Renewable Energy Laboratory (NREL) offers a suite of laboratory analytical procedures (LAPs) for biomass analysis.
  • Understanding compositional differences in various sorghum types (grain, forage, sweet, biomass) is essential for optimizing their use.

Purpose of the Study:

  • To describe the application of NREL's LAPs for detailed characterization of sorghum [Sorghum bicolor (L.) Moench].
  • To highlight the importance of specific analytical steps for differentiating carbohydrates, particularly glucose from starch and glucan from cellulose.
  • To provide a standardized methodology for analyzing diverse sorghum varieties.

Main Methods:

  • Utilized NREL's established laboratory analytical procedures (LAPs) for comprehensive feedstock analysis.
  • Included sample preparation, determination of moisture, ash, protein, extractives, and carbohydrates (sucrose, starch, glucan, xylan, etc.).
  • Focused on specialized extraction and hydrolysis techniques to accurately quantify glucose and glucan, especially in non-structural carbohydrate-rich sorghum.

Main Results:

  • Demonstrated the capability of NREL LAPs to provide detailed compositional data for various sorghum types.
  • Successfully differentiated glucose derived from starch versus glucan from cellulose.
  • Quantified key components including ash, protein, moisture, extractives, and various carbohydrates and lignin.

Conclusions:

  • NREL's LAPs are effective for detailed sorghum feedstock characterization.
  • Accurate carbohydrate analysis, especially distinguishing starch and glucan, is vital for biomass utilization.
  • The described procedures offer a robust framework for assessing sorghum's potential in biofuel and bio-based product development.