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MicroRNA-145 performs as a tumor suppressor in human esophageal squamous cell carcinoma by targeting phospholipase C

Chun Tang1,2, Jin-Yuan He3, Chao Yu4

  • 1Department of Nephrology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, P.R. China.

Journal of Cellular Biochemistry
|January 18, 2019
PubMed

Insights

MicroRNA-145 (miR-145) is downregulated in esophageal squamous cell carcinoma (ESCC). Restoring miR-145 suppresses ESCC cell proliferation and migration by targeting PLCE1, indicating its tumor suppressor role.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Esophageal squamous cell carcinoma (ESCC) is a prevalent cancer in China.
  • MicroRNA-145 (miR-145) is frequently downregulated in various cancers, suggesting a potential role in tumorigenesis.

Purpose of the Study:

  • To investigate the expression and function of miR-145 in ESCC.
  • To identify the molecular targets of miR-145 in ESCC.
  • To elucidate the therapeutic potential of miR-145 in ESCC.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were used to assess miR-145 and PLCE1 expression.
  • Bioinformatic tools predicted miR-145 targets.
  • MTT and wound healing assays evaluated the effects of miR-145 on ESCC cell proliferation and migration.

Main Results:

  • miR-145 expression was significantly decreased in ESCC tissues and inversely correlated with invasion depth and TNM stage.
  • Phospholipase C epsilon 1 (PLCE1) was identified as a direct target of miR-145, with inverse expression correlation observed in ESCC tissues.
  • Overexpression of miR-145 inhibited ESCC cell proliferation and migration, an effect partially reversed by PLCE1 re-expression.

Conclusions:

  • miR-145 functions as a tumor suppressor in ESCC.
  • Targeting PLCE1 is a key mechanism through which miR-145 exerts its tumor-suppressive effects in ESCC.
  • miR-145 represents a potential therapeutic target for esophageal squamous cell carcinoma.
Keywords:
esophageal squamous cell carcinomamicroRNA-145migrationphospholipase C epsilon 1proliferation

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