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Updated: Jan 30, 2026

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells
Published on: May 5, 2023
Amniotic membrane as a biological scaffold for dental pulp stem cell transplantation in ocular surface reconstruction
Babyla Geraldes Monteiro1,2, Renata Ruoco Loureiro3, Priscila Cardoso Cristovam3
1Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
Purpose:
To evaluate the ability of human immature dental pulp stem cells, which are mesenchymal stem cells of neural crest origin, to differentiate into the corneal epithelium for purposes of corneal transplantation and tissue engineering when cultured on de-epithelized amniotic membranes.
Methods:
We compared the immunophenotypes (ABCG2, K3/12, and vimentin) of cells grown on amniotic membranes or plastic surfaces under serum-free conditions or in culture media containing serum or serum replacement components.
Results:
Immature dental pulp stem cells grown on amniotic membranes under basal conditions are able to maintain their undifferentiated state. Our data also suggest that the culture medium used in the present work can modulate the expression of immature dental pulp stem cell markers, thus inducing epithelial differentiation of these cells in vitro.
Conclusions:
Our results suggest that the amniotic membrane is a good choice for the growth and transplantation of mesenchymal stem cells, particularly immature dental pulp stem cells, in clinical ocular surface reconstruction.
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