Parabacteroides acidifaciens sp. nov., isolated from human faeces

Area of Science:

• Microbiology
• Bacterial Taxonomy
• Genomics

Background:

• Human gut microbiota harbors diverse anaerobic bacteria.
• Accurate taxonomic classification is crucial for understanding microbial roles.

Purpose of the Study:

• To characterize a novel anaerobic bacterial strain isolated from human faeces.
• To determine the phylogenetic and chemotaxonomic properties of strain 426-9^T.
• To propose a new species within the Parabacteroides genus.

Main Methods:

• Polyphasic taxonomic analysis including phenotypic, chemotaxonomic, and phylogenetic methods.
• 16S rRNA gene sequence analysis for phylogenetic placement.
• Genome sequencing and analysis.

Main Results:

• Strain 426-9^T is a Gram-negative, non-motile, anaerobic rod.
• Optimal growth occurs at 37-40°C and pH 6.0-8.0.
• Phylogenetic analysis places strain 426-9^T within the Parabacteroides genus, with 91.0% 16S rRNA gene similarity to Parabacteroides distasonis.
• The draft genome size is 5.15 Mb with a G+C content of 45.9%.

Conclusions:

• Strain 426-9^T represents a novel species, Parabacteroides acidifaciens sp. nov.
• This finding contributes to the understanding of bacterial diversity in the human gut.