Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid microwave fixation--a comparative morphometric study.

K Kayser1, H Stute, J Lübcke

  • 1Department of Pathology, Hospital for Thoracic Diseases, Heidelberg, FR Germany.

The Histochemical Journal
|June 1, 1988
PubMed
Summary

Microwave fixation using Tris buffer offers superior lung tissue morphology compared to conventional formalin fixation. This time-saving method provides comparable results without environmental hazards.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Evisceration of intestines following abdominal stab wounds: epidemiology and clinical aspects of emergency room management].

Der Unfallchirurg·2013
Same author

The galactoside-specific lectin from mistletoe as biological response modifier.

International journal of oncology·2011
Same author

Prognostic relevance of detection of ligands for vertebrate galectins and a Lewis(Y)-specific monoclonal antibody.

International journal of oncology·2011
Same author

Disease type-associated increases of the plasma levels and ligand expression for natural alpha- or beta-galactoside-binding immunoglobulin G subfractions in patients with lung cancer.

International journal of oncology·2011
Same author

Lectin-triggered superoxide/h(2)o (2) and granule enzyme release from cells.

Methods in molecular medicine·2011
Same author

MHC class II tetramer guided detection of Mycobacterium tuberculosis-specific CD4+ T cells in peripheral blood from patients with pulmonary tuberculosis.

Scandinavian journal of immunology·2007

Area of Science:

  • Histopathology
  • Biotechnology
  • Medical Devices

Background:

  • Conventional formalin fixation is a standard but time-consuming method for preserving tissue morphology.
  • Microwave-assisted tissue fixation offers potential for faster processing and improved morphological detail.
  • Optimizing fixation protocols is crucial for accurate histopathological diagnosis.

Purpose of the Study:

  • To evaluate microwave-assisted fixation using various solutions for human lung tissue.
  • To compare the morphological quality of microwave-fixed tissues with conventional formalin fixation.
  • To assess nuclear morphometry as an objective measure of fixation quality.

Main Methods:

  • Human lung tissue samples (healthy, carcinoma, lymph nodes) were fixed using microwave irradiation with Tris buffer, formalin, NaCl, DMSO, acetone, methanol, and glutaraldehyde.

Related Experiment Videos

  • Control samples were fixed with conventional 7% buffered formalin for 24 hours.
  • Tissues were embedded in paraffin, stained with hematoxylin and eosin, and examined via light microscopy.
  • Nuclear morphometry (minimum diameter and area) was measured for 100 nuclei per specimen.

    • Main Results:

    • Microwave fixation with Tris buffer yielded the best morphological results, enhancing nuclear chromatin contrast and interstitial lung capillary visualization.
    • No statistically significant differences were observed in nuclear area or diameter across different fixation groups.
    • Histomorphological quality with microwave-Tris buffer fixation was superior to conventional formalin fixation.

    Conclusions:

    • Microwave fixation using Tris buffer is a rapid and effective alternative to conventional formalin fixation for lung tissue.
    • This method provides excellent morphological detail comparable to, and in some aspects better than, traditional methods.
    • The Tris buffer microwave fixation technique is environmentally friendlier than formalin fixation.