Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

10.6K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
10.6K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

8.4K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
8.4K
MicroRNAs01:22

MicroRNAs

24.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.2K
MicroRNAs01:22

MicroRNAs

4.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.0K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

26.4K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
26.4K
Regulated Protein Degradation02:58

Regulated Protein Degradation

8.8K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel CHD7 Variants Associated with an Expanded Orofacial and Dental Phenotype.

European journal of dentistry·2026
Same author

Exercise-induced lactic acidemia associated with a SLC16A13 biallelic variant.

NPJ genomic medicine·2026
Same author

Response to: "Beyond cardiotoxicity risk stratification: the paradoxical reverse association of age, BMI, and cancer type".

Heart and vessels·2026
Same author

Splenic rupture secondary to pegfilgrastim in a healthy allogeneic peripheral blood hematopoietic stem cell donor.

International journal of hematology·2026
Same author

Isoform-specific m6A deposition and coordinated splicing shape mammalian transcriptome evolution.

Nature communications·2026
Same author

Protocol for characterization of spatiotemporal network dynamics in cortical and hippocampal assembloids.

STAR protocols·2026

Related Experiment Video

Updated: Jan 30, 2026

Studying Wnt Signaling During Patterning of Conducting Airways
13:00

Studying Wnt Signaling During Patterning of Conducting Airways

Published on: October 16, 2016

7.8K

MicroRNAs control eyelid development through regulating Wnt signaling.

Takahiro Nagai1,2, Supaluk Trakanant1, Maiko Kawasaki1,3

  • 1Division of Oral Anatomy, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|January 18, 2019
PubMed
Summary

MicroRNAs (miRNAs) are essential for normal eyelid development. Their absence causes the eye-open at birth phenotype by disrupting Wnt signaling pathways crucial for morphogenesis.

Keywords:
BmpFgfShhWntdicereyelid developmentmesenchymemicroRNA

More Related Videos

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.7K
Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

1.9K

Related Experiment Videos

Last Updated: Jan 30, 2026

Studying Wnt Signaling During Patterning of Conducting Airways
13:00

Studying Wnt Signaling During Patterning of Conducting Airways

Published on: October 16, 2016

7.8K
Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.7K
Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

1.9K

Area of Science:

  • Developmental Biology
  • Molecular Genetics
  • Signaling Pathways

Background:

  • Organogenesis relies on precise gene expression timing and levels.
  • MicroRNAs (miRNAs) are key regulators of gene expression, but their role in organogenesis is not fully elucidated.
  • Mammalian eyelid fusion and separation are critical developmental processes; failure results in the eye-open at birth (EOB) phenotype in mice.

Purpose of the Study:

  • To investigate the role of miRNAs in mammalian eyelid development.
  • To understand the molecular mechanisms underlying the eye-open at birth (EOB) phenotype in miRNA processing mutants.

Main Methods:

  • Conditional deletion of Dicer in mesenchymal cells (Dicer fl/fl ;Wnt1Cre) to study miRNA function.
  • Analysis of Wnt, Fgf, Shh, and Bmp signaling pathways in Dicer mutants.
  • Investigating epithelial-mesenchymal interactions during eyelid development.

Main Results:

  • Mesenchymal Dicer deletion leads to the EOB phenotype.
  • Up-regulation of Wnt signaling is identified as a cause of the EOB phenotype in Dicer mutants.
  • Down-regulation of Fgf, Shh, and Bmp signaling occurs secondary to Wnt pathway alterations, highlighting an inverse relationship between Fgf and Wnt signaling.
  • Wnt, Shh, and Fgf signaling mediate crucial epithelial-mesenchymal interactions in eyelid development.

Conclusions:

  • MicroRNAs are critical regulators of eyelid development, primarily through the modulation of Wnt signaling.
  • The study elucidates the molecular basis of the EOB phenotype, linking miRNA processing to key developmental signaling pathways.
  • Findings provide insights into the complex interplay of signaling pathways governing mammalian organogenesis.