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Related Experiment Videos

Ultrastructural localization of immunoreactivity in the developing piriform cortex.

R E Westenbroek1, L E Westrum, A E Hendrickson

  • 1Department of Neurological Surgery, University of Washington, Seattle 98195.

The Journal of Comparative Neurology
|August 15, 1988
PubMed
Summary

This study reveals how cholecystokinin (CCK) and glutamic acid decarboxylase (GAD) labeled synapses develop in the rat piriform cortex. Inhibitory GABA synapses mature before excitatory CCK synapses, showing a developmental gradient.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • The piriform cortex (PC) is crucial for olfactory processing.
  • Understanding synaptic development is key to deciphering neural circuit formation.
  • Immunohistochemistry allows visualization of specific neurotransmitter systems during development.

Purpose of the Study:

  • To investigate the ultrastructural basis of immunoreactivity patterns in synaptic structures during piriform cortex development.
  • To map the developmental timeline and characteristics of cholecystokinin (CCK) and glutamic acid decarboxylase (GAD) labeled synapses in rat PC layers I and II.

Main Methods:

  • Utilized antisera against CCK and GAD in rat pups at various postnatal days (PN) and in adults.
  • Examined labeled axons, terminals, and synaptic contacts using electron microscopy.

Related Experiment Videos

  • Quantified the distribution, morphology, and frequency of GAD- and CCK-positive profiles.

    • Main Results:

    • GAD-positive terminals and contacts increased in layer I after PN 6, with adult features by PN 21.
    • CCK-labeled terminals and contacts increased significantly between PN 2 and 9, with mature morphology by PN 13.
    • Inhibitory (GABA) synapses established earlier than potentially excitatory CCK synapses; a deep-to-superficial synaptogenesis gradient observed for GAD terminals.

    Conclusions:

    • Synaptic immunoreactivity precedes synapse formation and presynaptic specialization maturation.
    • GAD and CCK terminals share similar vesicle morphology and membrane specializations in mature synapses.
    • Developmental patterns suggest critical periods for synaptogenesis in the piriform cortex.