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Related Experiment Video

Updated: Jan 30, 2026

In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays
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DnaJ chaperones contribute to canalization.

Samantha Hughes1, Inge Vrinds1, Joris de Roo1

  • 1HAN BioCentre, HAN University of Applied Science, Isnstitute of Applied Biosciences and Chemistry, Nijmegen, The Netherlands.

Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology
|January 18, 2019
PubMed
Summary

Developmental canalization, or robustness, is influenced by DnaJ chaperones. This study found that multiple DnaJ genes, including those not ER-associated, play conserved roles in buffering against environmental and genetic stress across animals.

Keywords:
Caenorhabditis elegansDnaJsdevelopmental robustnesslifespanseam cell

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Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Canalization describes biological robustness to perturbations.
  • The molecular basis for environmental canalization (EC) versus genetic canalization is not fully understood.
  • Conserved roles of endoplasmic reticulum (ER)-associated DnaJ genes in developmental buffering have been recently discovered.

Purpose of the Study:

  • To investigate the conserved role of DnaJ chaperones in canalization across Metazoa.
  • To determine if ER-associated DnaJ genes are involved in environmental canalization (EC).
  • To identify specific DnaJ genes contributing to developmental robustness.

Main Methods:

  • Surveyed ER-associated DnaJ chaperones in Caenorhabditis elegans.
  • Quantified phenotypic variance and mean of seam cell counts using RNA interference (RNAi) knockdown.
  • Assessed DnaJ involvement under three different temperature conditions.

Main Results:

  • Seven of eight ER-associated DnaJ genes were implicated in EC or microenvironmental canalization.
  • Two non-ER-associated DnaJ genes (DNAJC2/dnj-11 and DNAJA2/dnj-19) also contributed to canalization.
  • Most identified buffering DnaJ chaperones also influenced lifespan.

Conclusions:

  • A diverse set of DnaJ chaperones, beyond ER-associated ones, are involved in canalization.
  • These DnaJ chaperones are integral components of the complex developmental buffering system.
  • The findings suggest conserved mechanisms of developmental robustness across the animal kingdom.