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Updated: Jan 30, 2026

Characterizing Extracellular Vesicles from Biological Fluids
Published on: February 28, 2025
Extracellular Vesicle Quantification and Characterization: Common Methods and Emerging Approaches
Thomas A Hartjes1, Serhii Mytnyk2, Guido W Jenster3
1Department of Pathology, Erasmus Optical Imaging Centre, Erasmus MC, 3015 GE Rotterdam, The Netherlands. t.hartjes@erasmusmc.nl.
Abstract:
Extracellular vesicles (EVs) are a family of small membrane vesicles that carry information about cells by which they are secreted. Growing interest in the role of EVs in intercellular communication, but also in using their diagnostic, prognostic and therapeutic potential in (bio) medical applications, demands for accurate assessment of their biochemical and physical properties. In this review, we provide an overview of available technologies for EV analysis by describing their working principles, assessing their utility in EV research and summarising their potential and limitations. To emphasise the innovations in EV analysis, we also highlight the unique possibilities of emerging technologies with high potential for further development.
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