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Related Experiment Videos

LexA repressor induces operator-dependent DNA bending.

R Lloubès1, M Granger-Schnarr, C Lazdunski

  • 1Centre de Biologie et de Biochimie Moléculaire, Marseille, France.

Journal of Molecular Biology
|December 20, 1988
PubMed
Summary

LexA repressor binding causes significant DNA bending in the caa operator sequence of Escherichia coli. This bending is intrinsic to the caa operator, unlike the recA operator.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • The SOS DNA damage response in Escherichia coli is regulated by the LexA repressor.
  • LexA controls the expression of genes involved in DNA repair, including the caa gene encoding colicin A.

Purpose of the Study:

  • To investigate the DNA bending induced by LexA binding to its operator sequences.
  • To compare the bending of the caa operator versus the recA operator upon LexA interaction.

Main Methods:

  • Gel electrophoresis was used to analyze DNA bending.
  • A series of DNA fragments with varying caa operator positions were studied.

Main Results:

  • LexA binding induced substantial DNA bending specifically at the caa operator.

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  • No significant DNA bending was observed upon LexA binding to the recA operator.
  • The caa operator exhibited intrinsic DNA bendability, independent of LexA binding, due to flanking thymine tracts.

    • Conclusions:

    • The caa operator's sequence, with flanking thymine tracts, promotes intrinsic DNA bending.
    • LexA binding to the caa operator enhances this inherent bend, while the recA operator does not induce bending.
    • DNA bending is a key feature of LexA-operator interactions, particularly for the caa operator.