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MicroRNA-24-3p regulates Hodgkin's lymphoma cell proliferation, migration and invasion by targeting DEDD
Jing Wang1, Kai Yin2, Xianping Lv1
1Department of Blood Transfusion, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
Abstract:
Hodgkin's lymphoma (HL) is a common hematologic tumor, and the incidence is increasing. At present, it is considered that miRNAs are closely related to HL. Substantial attention has been paid to the effects of miRNA on the pathophysiological process of HL. This study was focused on the potential role of miR-24-3p in HL by targeting DEDD. The reverse transcription-quantitative PCR (RT-qPCR) results demonstrated that miR-24-3p expression was highly elevated and DEDD expression reduced inversely in HL tissues compared to adjacent tissues. According to the results of CKK-8 assays, miR-24-3p was able to accelerate HL cell proliferation. In addition, the results of the Transwell assays also indicated that miR-24-3p promoted the invasion and migration abilities of HL cells. Moreover, the results demonstrated that miR-24-3p inhibited DEDD expression. Hence, the present study revealed that miR-24-3p could accelerate HL development through inhibiting DEDD.
Insights
MicroRNA-24-3p (miR-24-3p) is elevated in Hodgkin's lymphoma (HL) and promotes cancer cell growth, invasion, and migration. This study reveals miR-24-3p accelerates HL development by inhibiting the DEDD gene.
Area of Science:
- Hematologic oncology
- Molecular biology
- Cancer research
Background:
- Hodgkin's lymphoma (HL) is a common hematologic malignancy with increasing incidence.
- MicroRNAs (miRNAs) are implicated in the pathophysiology of HL.
- Understanding miRNA roles is crucial for HL research.
Purpose of the Study:
- To investigate the role of miR-24-3p in Hodgkin's lymphoma.
- To explore the relationship between miR-24-3p and its target gene, DEDD.
- To elucidate the mechanism by which miR-24-3p influences HL progression.
Main Methods:
- Reverse transcription-quantitative PCR (RT-qPCR) to measure miR-24-3p and DEDD expression.
- Cell Counting Kit-8 (CCK-8) assays to assess cell proliferation.
- Transwell assays to evaluate cell invasion and migration.
Main Results:
- miR-24-3p expression was significantly upregulated in HL tissues compared to adjacent tissues.
- DEDD expression was inversely correlated with miR-24-3p levels in HL tissues.
- miR-24-3p accelerated HL cell proliferation, invasion, and migration.
- miR-24-3p was confirmed to inhibit DEDD expression.
Conclusions:
- miR-24-3p plays a significant role in promoting Hodgkin's lymphoma development.
- The mechanism involves the inhibition of DEDD by miR-24-3p.
- miR-24-3p represents a potential therapeutic target for Hodgkin's lymphoma.