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RNA sequence analysis data of Peronospora destructor maintained on onions
Kazuki Fujiwara1, Takashi Fujikawa2, Akira Kawakami1
1Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization (NARO), Suya 2421, Koshi, Kumamoto 861-1192, Japan.
Data in Brief
|January 19, 2019
Summary
This study sequences the transcriptome of Peronospora destructor, the oomycete causing onion downy mildew. This provides a foundational resource for understanding the molecular mechanisms of this important plant pathogen.
Area of Science:
- Plant Pathology
- Molecular Biology
- Genomics
Background:
- Peronospora destructor causes downy mildew, a significant disease impacting onion yield and quality globally.
- While epidemiological knowledge is established, the molecular mechanisms of P. destructor infection remain largely unexplored.
Purpose of the Study:
- To generate a foundational transcriptome dataset for Peronospora destructor.
- To enable future research into the molecular interactions during onion downy mildew development.
Main Methods:
- Isolation of RNA from P. destructor sporangia and sporangiophores.
- Next-generation sequencing using an Ion PGM platform.
- Transcriptome shotgun assembly to generate sequence contigs.
Main Results:
- Successfully assembled 2335 contigs from the P. destructor transcriptome.
- Generated a valuable genomic resource for the pathogen, accessible via NCBI BioProject PRJNA391849.
Conclusions:
- The generated transcriptome data provides a crucial resource for advancing the molecular understanding of P. destructor.
- Facilitates future investigations into pathogen virulence and host-pathogen interactions in onion downy mildew.
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