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Updated: Jan 30, 2026
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Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma
Published on: April 11, 2018
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Making NSC and Neurons from Patient-Derived Tissue Samples.
Odity Mukherjee1, Shubhra Acharya2, Mahendra Rao2,3
1Institute of Stem Cells and Regenerative Medicine (inStem), Bangalore, India. omukherjee@ncbs.res.in.
Methods in Molecular Biology (Clifton, N.J.)
|January 19, 2019
Summary
Human pluripotent stem cells (HPSCs) offer a unique model for studying brain development and disorders. This research details methods for differentiating HPSCs into neural stem cells (NSCs) and neurons for disease modeling and drug screening.
Area of Science:
- Neuroscience
- Stem Cell Biology
- Developmental Biology
Background:
- The human brain's complexity and associated disorders necessitate advanced research models.
- While model organisms offer insights, human-specific features require dedicated study.
- Human pluripotent stem cells (HPSCs) provide a valuable resource for understanding human brain development.
Purpose of the Study:
- To detail the differentiation of HPSCs into neural stem cells (NSCs) and neurons.
- To review in vivo neural differentiation mechanisms and their in vitro recapitulation.
- To provide a guide for deriving region- and lineage-specific NSCs and neurons.
Main Methods:
- Utilizing HPSCs for neural differentiation.
- Recapitulating in vivo neural differentiation mechanisms in vitro.
- Employing a core protocol for NSC and mature neuron derivation.
Main Results:
- Established methods for deriving NSCs and neurons from HPSCs.
- Detailed key steps within the core differentiation protocol.
- Provided a transcription factor and morphogen map for guided differentiation.
Conclusions:
- HPSC differentiation is a powerful tool for studying human neural development.
- Derived NSCs and neurons can be used for disease modeling and therapeutic screening.
- The provided map facilitates the generation of specific neural cell types.
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