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Updated: Jan 30, 2026

Homing of Hematopoietic Cells to the Bone Marrow
Published on: March 18, 2009
Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation
Cang Chen1, Michael J Guderyon1, Guo Ge1
1Department of Medicine, UT Health Science Center at San Antonio, San Antonio, TX, USA.
Abstract:
Lentiviral vectors are increasingly used as efficient gene transfer tools in the experimental and clinical gene therapy treatment of acquired and inherited genetic diseases. Hematopoietic stem cells (HSCs) are characterized by the capacity for self-renewal, as well as multi-lineage differentiation and maintenance of the lymphohematopoietic system throughout life. As such, HSC transplantation (HSCT) has proven to be a powerful therapeutic modality for the treatment of both malignant and nonmalignant disorders. Transduction of lentiviral vectors into HSCs may offer long-term stable expression of a therapeutic gene in both preclinical and clinical settings. The purpose of this chapter is to describe an optimized procedure for lentiviral transduction of mouse HSCs followed by HSCT.
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