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Related Concept Videos

Overview of Microscopy Techniques01:22

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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Brick-cutting techniques involve various tools and methods to shape bricks for construction. A mason's hammer with a chisel-pointed end is used for basic shaping through sharp, precise strikes. For more complex shapes requiring higher precision, a power saw with a water-cooled diamond blade is used.
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Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...
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OverviewStaining techniques in microscopy enhance the visualization of microorganisms by increasing contrast and allowing the differentiation of cellular structures. Simple staining is one of the fundamental methods used to observe the basic morphological characteristics of microorganisms, including their size, shape, and arrangement. This method relies on the application of a single dye to stain the entire cell, producing a clear contrast between the cell and the background.FixationFixation is...
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Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
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Techniques of Laparoscopic Right Posterior Sectionectomy: Glissonian Approach and a Parenchymal Transection Technique
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Laparoscopic entry techniques.

Gaity Ahmad1, Jade Baker, John Finnerty

  • 1Department of Obstetrics and Gynaecology, Pennine Acute Hospitals NHS Trust, Manchester, UK.

The Cochrane Database of Systematic Reviews
|January 19, 2019
PubMed
Summary
This summary is machine-generated.

Current evidence is insufficient to recommend one laparoscopic entry technique over another. Direct trocar entry may reduce failed entries compared to Veress needle entry, but more research is needed.

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Area of Science:

  • Surgical Procedures
  • Minimally Invasive Surgery
  • Patient Safety

Background:

  • Laparoscopic surgery complications often stem from initial abdominal entry.
  • Life-threatening risks include visceral and vascular injuries.
  • Optimal laparoscopic entry techniques lack a clear consensus.

Purpose of the Study:

  • To evaluate the benefits and risks of various laparoscopic entry techniques.
  • Comparison of techniques in gynecological and non-gynecological surgery.

Main Methods:

  • Systematic review of randomized controlled trials (RCTs).
  • Searched multiple databases including Cochrane, MEDLINE, and Embase.
  • Assessed primary outcomes like mortality, vascular/visceral injury, and failed entry.

Main Results:

  • 57 RCTs (9865 participants) evaluated 25 entry techniques.
  • Insufficient evidence to differentiate major complications between techniques.
  • Direct trocar entry showed reduced failed entries versus Veress needle (moderate-quality evidence).

Conclusions:

  • Overall evidence is insufficient to favor one laparoscopic entry technique.
  • Limitations include low event rates, small sample sizes, and risk of bias.
  • Further high-quality research is needed to establish optimal entry methods.