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Plasmonic Nanosensor Array for Multiplexed DNA-based Pathogen Detection.

David Zopf1, Angelina Pittner1, André Dathe1,2

  • 1Leibniz Institute of Photonic Technology (IPHT) Jena , Member of the Leibniz Research Alliance - Leibniz Health Technologies , Albert-Einstein-Straße 9 , 07745 Jena , Germany.

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Summary

This study presents a novel optical biosensor using gold nanoparticles for detecting DNA. The sensor enables sensitive, reversible, and multiplexed detection of fungal pathogen DNA sequences.

Keywords:
DNA detectionFourier-transform-imaging spectroscopyLSPR sensingplasmonic arrayplasmonic nanoparticles

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Area of Science:

  • Nanotechnology
  • Biotechnology
  • Spectroscopy

Background:

  • Molecular binding events are crucial in biological processes.
  • Developing sensitive and specific biosensors is essential for diagnostics.

Purpose of the Study:

  • To introduce a plasmonic nanoparticle-based optical biosensor.
  • To demonstrate its capability for monitoring molecular binding events, specifically DNA hybridization.

Main Methods:

  • Utilized spotted gold nanoparticle arrays as the sensing platform.
  • Functionalized nanoparticle spots with capture DNA sequences.
  • Detected binding events via changes in localized surface plasmon resonance (LSPR) peak wavelength.
  • Employed gold nanoparticle labels to enhance signal and HCl for reversibility.

Main Results:

  • Successfully monitored molecular binding events through spectroscopic detection of LSPR peak shifts.
  • Achieved enhanced signal detection using gold nanoparticle labels.
  • Demonstrated reversible binding using chemical treatment.
  • Showcased multiplexed detection and identification of multiple fungal pathogen DNA sequences on a single sensor array.

Conclusions:

  • The developed plasmonic nanoparticle optical biosensor is effective for monitoring DNA binding.
  • The sensor offers sensitivity, reversibility, and multiplexing capabilities for pathogen detection.