Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

18.3K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
18.3K
Stereotypes, Prejudice, and Discrimination02:55

Stereotypes, Prejudice, and Discrimination

95.4K
Humans are very diverse and although we share many similarities, we also have many differences. The social groups we belong to help form our identities (Tajfel, 1974). These differences may be difficult for some people to reconcile, which may lead to prejudice toward people who are different. Prejudice is a negative attitude and feeling toward an individual based solely on one’s membership in a particular social group (Allport, 1954; Brown, 2010). Prejudice is common against people who...
95.4K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

40.8K
Overview
40.8K
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

5.1K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
5.1K
Generalization, Discrimination, and Extinction01:24

Generalization, Discrimination, and Extinction

1.4K
Generalization, discrimination, and extinction are key concepts in operant conditioning that influence how behaviors are learned and maintained.
Generalization occurs when a behavior reinforced in one context is performed in similar situations. For instance, a student who studies diligently for calculus and receives excellent grades might apply the same study habits to psychology and history, expecting similar results. Generalization shows how learning in one setting can influence behavior in...
1.4K
Nucleic Acids and Nucleotides01:20

Nucleic Acids and Nucleotides

14.4K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and have instructions for its functioning. The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).
Deoxyribonucleic Acid (DNA)
DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and the organelles such as chloroplasts and mitochondria....
14.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Principle-based multiphysics simulation for 3D bioprinting systems: modelling inkjet, extrusion, and DLP processes.

Biofabrication·2026
Same author

Advances in 3D printed blood-brain barrier models.

Biofabrication·2026
Same author

Effects of Liposome Composition and Size on Protein Corona Formation: Competitive Replacement of Abundant Proteins by High-Affinity Proteins on the Liposome Surface.

Advanced healthcare materials·2026
Same author

Comprehensive Evaluation of the Genotoxic Potential of Food Additive Titanium Dioxide in Human Intestinal Cell Systems.

International journal of molecular sciences·2025
Same author

GENRISE-induced superior extracellular vesicles for scalable therapeutic cargo delivery.

Science advances·2025
Same author

A Dual-Responsive Starch Nanoplatform for Triggered Iodine Release against <i>Helicobacter pylori</i>.

ACS applied materials & interfaces·2025

Related Experiment Video

Updated: Jan 30, 2026

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles
08:31

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles

Published on: March 20, 2019

8.0K

Alpha-Hederin Nanopore for Single Nucleotide Discrimination.

Ki-Baek Jeong1, Ke Luo1, Hwankyu Lee2

  • 1Graduate School of Biotechnology and Department of Food Science and Biotechnology , Kyung Hee University , Yongin 17104 , Republic of Korea.

ACS Nano
|January 19, 2019
PubMed
Summary

Researchers developed a novel nonproteinaceous nanopore from alpha-hederin for biomolecule analysis. This synthetic pore shows potential for DNA sequencing by discriminating nucleotides and single-stranded DNA (ssDNA).

Keywords:
alpha-hederindiscriminationnanoporeself-assemblysingle nucleotidesize-tunable

More Related Videos

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

9.3K
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

12.1K

Related Experiment Videos

Last Updated: Jan 30, 2026

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles
08:31

A Closed-Type Wireless Nanopore Electrode for Analyzing Single Nanoparticles

Published on: March 20, 2019

8.0K
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

9.3K
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

12.1K

Area of Science:

  • Biophysics
  • Nanotechnology
  • Molecular Biology

Background:

  • Biological and synthetic nanopores are crucial for high-throughput analysis of individual biomolecules.
  • Protein-based nanopores offer high sensitivity and reproducibility but have fixed dimensions, limiting adaptability.
  • Existing nanopore technology faces challenges in customizability for diverse applications.

Purpose of the Study:

  • To develop a novel, nonproteinaceous nanopore inspired by the cytotoxic effects of alpha-hederin.
  • To investigate the pore-forming mechanism of alpha-hederin in lipid membranes.
  • To demonstrate the potential of this new nanopore for biomolecule discrimination and DNA sequencing.

Main Methods:

  • Formation of nonproteinaceous nanopores using alpha-hederin in cholesterol-rich lipid membranes.
  • Investigation of the pore-forming mechanism and control of pore formation rate via lipid partitioning.
  • Electrophysiological measurements to assess nanopore characteristics and molecule translocation.

Main Results:

  • Spontaneous formation of alpha-hederin nanopores in lipid membranes was achieved.
  • The mechanism of alpha-hederin pore formation in cholesterol-rich membranes was proposed.
  • Alpha-hederin nanopores successfully discriminated between single-stranded DNA (ssDNA) homopolymers and individual nucleotides.

Conclusions:

  • Alpha-hederin forms functional, nonproteinaceous nanopores in lipid membranes.
  • The pore-forming rate can be modulated using lipid partitioning strategies.
  • These novel nanopores show promise as a versatile tool for DNA sequencing and other molecular analysis applications.