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Updated: Jan 30, 2026

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
Published on: June 16, 2022
Improved viability of spray-dried Lactobacillus bulgaricus sp1.1 embedded in acidic-basic proteins treated with
Pimin Gong1, Wei Di1, Huaxi Yi2
1Department of Food Science and Engineering, Harbin Institute of Technology, Harbin 150000, China.
Abstract:
Spray drying is a fast and low-cost method of drying lactic acid bacteria, but it features a low survival rate. A pre-treatment was designed to increase the survival by embedding bacteria in basic or acidic proteins with the principle of opposite charges attraction. Transglutaminase (TGase)-induced cross-linking then tightened the cell-protein complexes. The results showed that mixing of basic proteins with acidic proteins and TGase treatment increased the survival rates of spray-dried Lactobacillus bulgaricus sp1.1. 84.78% of cells in the TGase-treated milk basic protein (MBP)-soy protein isolate (SPI) bacterial solution remained after drying. Positively charged MBP adhered to more bacteria (negative charge) and formed tight closed loop-shaped complexes with SPI (negative charge) by electrostatic attraction, and TGase enhanced this attraction by strengthening of the hydrogen, CO and CN bonds and exposing the inner α-helix. Imbedding bacteria in TGase-treated acidic-basic protein can increase efficiency of the current formulation.
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