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Microplate-based surface area assay for rapid phenotypic antibiotic susceptibility testing.

Kelly Flentie1, Benjamin R Spears1, Felicia Chen1

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A new method provides rapid phenotypic antibiotic susceptibility testing (AST) in 5 hours, improving patient outcomes and combating antibiotic resistance. This surface-binding technique enhances accuracy and allows testing of more antibiotics simultaneously.

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Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Infectious Diseases

Background:

  • Rapid antibiotic susceptibility testing (AST) is crucial for effective infectious disease treatment and antibiotic stewardship.
  • Conventional AST methods are slow (overnight) or limited in the number of antibiotics tested.
  • Existing rapid AST platforms have limitations in accuracy and antibiotic panel breadth.

Purpose of the Study:

  • To develop a novel, rapid phenotypic AST method enabling faster and broader antibiotic testing.
  • To improve the accuracy of AST measurements, especially for bacteria exhibiting morphological changes under antibiotic stress.

Main Methods:

  • A novel AST approach utilizing signal amplification of bacterial surfaces.
  • Phenotypic testing performed on standard microplates as an endpoint assay.
  • Measurement of bacterial replication through surface binding and signal amplification.

Main Results:

  • Achieved phenotypic AST results within 5 hours for non-fastidious bacteria.
  • Demonstrated accurate measurements of bacterial replication, accounting for filamentation or swelling.
  • Enabled parallel testing of a wider range of antibiotics compared to current automated systems.

Conclusions:

  • This novel surface signal amplification method offers rapid and accurate phenotypic AST.
  • The technology has the potential to significantly advance clinical practice by providing comprehensive antibiotic susceptibility data quickly.
  • Facilitates improved patient outcomes and supports antibiotic stewardship efforts.