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Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
Published on: September 20, 2016
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Construction of mini-chemostats for high-throughput strain characterization
David Bergenholm1, Guodong Liu1, David Hansson1
1Novo Nordisk Foundation Center for Biosustainability, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
Biotechnology and Bioengineering
|January 20, 2019
Summary
A new, low-cost mini-chemostat (MC) system enables high-throughput microbial research. This reproducible system provides stable cell cultivation for systems biology, reducing media and complexity compared to traditional chemostats.
Area of Science:
- Microbiology
- Systems Biology
- Biotechnology
Background:
- High-throughput systems biology requires robust microbial cultivation.
- Existing chemostats are often costly, complex, and media-intensive.
- There is a need for scalable, affordable chemostat systems.
Purpose of the Study:
- To develop a low-cost, scalable mini-chemostat (MC) system.
- To enable high-throughput cultivation of microorganisms.
- To validate the MC system's performance against established methods.
Main Methods:
- Developed a 16-reactor mini-chemostat (MC) system with 40 mL working volumes.
- Integrated dissolved oxygen, CO2, and pH sensors for environmental monitoring.
- Utilized RNA sequencing to compare yeast physiology grown in MC and DASGIP systems.
Main Results:
- The MC system successfully maintained stable, controlled cellular environments.
- Yeast physiology characterization revealed comparable results to a larger DASGIP system.
- Demonstrated reproducibility of the MC system across multiple experimental runs.
Conclusions:
- The developed MC system offers a cost-effective and reproducible solution for high-throughput microbial cultivation.
- This system facilitates large-scale systems biology research by providing reliable data.
- The MC system is scalable and adaptable for future experimental needs.
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