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Updated: Jan 30, 2026

Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans
Published on: July 3, 2020
Molecular characterization and expression patterns of Phenacoccus solenopsis (Hemiptera: Pseudococcidae) heat shock
Dan Liu1, Xiangli Dang1, Wei Song1
1Department of Entomology, College of Plant Protection, Anhui Agricultural University, Hefei, Anhui, China.
Abstract:
As a polyphagous insect, little is known at the molecular level about the effects of different host plants on physiological changes in Phenacoccus solenopsis. In this study, four heat shock protein (Hsp) genes (PsHsp60, PsHsp70, PsHsp90, and PsHsp20.7) were identified from the transcriptome of P. solenopsis. Analysis of Hsp expression levels revealed significant differences in Hsp gene expression levels in P. solenopsis fed on different host plants. In host conversion tests, the expression levels of PsHsp90 and PsHsp60 were upregulated after transfer of second instar nymphs from tomato to cotton. The expression levels of PsHsp70 and PsHsp20.7 were, respectively, significantly upregulated at 9 and 48 hr after transfer from tomato to Hibiscus. The results of this study aid molecular characterization and understanding of the expression patterns of Hsp genes during different developmental stages and host transfer of P. solenopsis.
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