Related Experiment Video

Updated: Jan 30, 2026

Analysis of Coronary Vessels in Cleared Embryonic Hearts
08:25

Analysis of Coronary Vessels in Cleared Embryonic Hearts

Published on: December 7, 2016

8.6K

Unexpectedly higher diazoxon hydrolysis by serum paraoxonase-1 in coronary heart disease

Bharti Mackness1, Mike Mackness1

  • 1Avda. Princip D'Espanya, 43892 Miami Playa, Tarragona, Spain.

Clinical Biochemistry
|January 20, 2019
PubMed

Insights

Serum paraoxonase-1 (PON1) activity toward diazoxon was significantly higher in individuals with coronary heart disease (CHD). This finding suggests potential diagnostic and mechanistic implications for PON1 in CHD, pending further research.

Area of Science:

  • Biochemistry
  • Cardiovascular Medicine
  • Genetics

Background:

  • Low serum paraoxonase-1 (PON1) activity is linked to coronary heart disease (CHD).
  • PON1 plays a role in hydrolyzing various substrates, but its specific role in CHD is not fully understood.
  • Investigating PON1 activity with specific substrates like diazoxon is crucial for understanding its association with CHD.

Purpose of the Study:

  • To investigate the rate of diazoxon hydrolysis by PON1 in a population with CHD.
  • To compare PON1 activity and genotypes between CHD patients and controls.
  • To explore the relationship between PON1 polymorphisms (PON1-55 and PON1-192) and diazoxon hydrolysis in CHD.

Main Methods:

  • A case-control study involving 410 subjects with CHD and 274 controls.
  • Measurement of PON1 activity using paraoxon and diazoxon substrates.
  • Determination of serum PON1 concentration and genotyping for PON1-55 and PON1-192 polymorphisms.

Main Results:

  • No significant differences in PON1-55 or PON1-192 genotype distribution between CHD patients and controls.
  • PON1 activity towards diazoxon (DIAZ) was significantly higher (+160%) in the CHD group compared to controls.
  • DIAZ activity showed significant differences across PON1-192 and PON1-55 genotypes in both populations, with specific orders observed for QQ > QR > RR and LL > LM > MM, respectively.

Conclusions:

  • The significantly higher diazoxon hydrolysis rate in CHD patients warrants further investigation.
  • Replication of these findings with other PON1 substrates and in diverse populations is essential.
  • These results may have significant diagnostic and mechanistic implications for understanding the role of PON1 in CHD.
Abstract

Keywords:
CHDDiazoxonHDLOxidationParaoxonase 1

More Related Videos

Author Spotlight: Innovative Technique for Coronary Angiography in Marginal Donors06:25

Author Spotlight: Innovative Technique for Coronary Angiography in Marginal Donors

Published on: July 12, 2024

850
Novel Percutaneous Approach for Deployment of 3D Printed Coronary Stenosis Implants in Swine Models of Ischemic Heart Disease06:39

Novel Percutaneous Approach for Deployment of 3D Printed Coronary Stenosis Implants in Swine Models of Ischemic Heart Disease

Published on: February 18, 2020

7.2K

Related Concept Videos

Hydrolysis of ATP01:08

Hydrolysis of ATP

The bonds of adenosine triphosphate (ATP) can be broken through the addition of water, releasing one or two phosphate groups in an exergonic process called hydrolysis. This reaction liberates the energy in the bonds for use in the cell—for instance, to synthesize proteins from amino acids.
If one phosphate group is removed, a molecule of ADP—adenosine diphosphate—remains, along with inorganic phosphate. ADP can be further hydrolyzed to AMP—adenosine...
81.3K
Hydrolysis01:15

Hydrolysis

Overview
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...
122.2K
Coronary Artery Disease I: Introduction01:30

Coronary Artery Disease I: Introduction

Coronary Artery Disease (CAD): An Overview with Scientific InsightsCoronary Artery Disease (CAD), often referred to as C-A-D, is a prevalent blood vessel disorder classified under the broader category of atherosclerosis. Atherosclerosis is a pathological process characterized by the hardening and narrowing of arteries due to the accumulation of atherosclerotic plaques. These plaques are composed of cholesterol, fatty substances, inflammatory cells, calcium, and fibrin, reducing blood flow to...
1.1K
Coronary Artery Disease II: Pathophysiology01:26

Coronary Artery Disease II: Pathophysiology

Coronary Artery Disease (CAD) originates from a series of events that impair the function of coronary arteries, the blood vessels responsible for delivering oxygen-rich blood to the heart muscle. The pathophysiology of CAD is closely linked to atherosclerosis, a chronic inflammatory and lipid-driven condition affecting the vascular endothelium.1. Endothelial DamageThe process begins with damage to the vascular endothelium, which serves as a protective barrier between the blood and the vessel...
428
Coronary Artery Disease V: Interprofessional Care01:27

Coronary Artery Disease V: Interprofessional Care

Interprofessional care for coronary artery disease includes pharmacological therapy and revascularization procedures.Pharmacological therapy for Coronary Artery Disease (CAD) aims to manage symptoms, prevent complications, and improve patient outcomes through various classes of medications:Antiplatelet Agents:Aspirin and Clopidogrel: These medications inhibit platelet aggregation, preventing blood clots, which is crucial for avoiding heart attacks and strokes. Doctors often prescribe these...
267
Rheumatic Heart Disease I: Introduction01:23

Rheumatic Heart Disease I: Introduction

Rheumatic heart disease or RHD is a chronic condition that results from rheumatic fever, causing permanent damage to the heart valves.Etiology and Risk FactorsIt primarily arises from rheumatic fever, an inflammatory disease that can develop after untreated or inadequately treated group A streptococcal (GAS) pharyngitis. Streptococcus spreads through direct contact with oral or respiratory secretions. While the bacteria are the causative agents, factors like malnutrition, overcrowding, poor...
519