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Updated: Jan 30, 2026
An In Vitro Model to Study the Effect of 5-Aminolevulinic Acid-mediated Photodynamic Therapy on Staphylococcus aureus Biofilm
Published on: April 16, 2018
Introduction of 5-aminolevulinic acid as a theranostics agent in dentistry
Sahar Mohammadpour Lashkari1, Hasan Kariminezhad1, Hossein Amani2
1Department of Physics, Faculty of Basic Sciences, Babol Noshirvani University of Technology, Babol, Po.Box: 47148-71167, Iran.
Objective:
The aim of this paper is to study the theranostic potential of 5-Aminolevulinic Acid (5-ALA)in dentistry.
Methods:
Photodynamic inactivation (PDI) and fluorescence spectroscopy of Streptococcus sanguis, and laser induced fluorescence (LIF) of several decayed teeth were performed using 5-ALA.
Results:
In the absence of 5-ALA, 15 min illumination of the bacteria by the means of an LED light source led to only 1.16% viability reduction. On the other hand, 5-ALA revealed remarkable dark toxicity at concentrations above 20 μM. Furthermore, the synergistic effects of 10 μM 5-ALA and illumination by the light source for 5 and 15 min intervals led respectivelyto0.74log10 and 1.69log10 reduction of viability. Also, fluorescence spectroscopy of the bacteria showed a direct relationship between emission line intensity at 620 nm and the concentration of 5-ALA. In dental experiments, following exposing tooth with 40 mM 5-ALA, a significant autofluorescence growth was observed just in the decayed parts.
Conclusion:
Based on the strong dual modality of 5-ALA to annihilate cariogenic bacteria through photodynamic inactivation and enhancing LIF intensity for identification of dental caries, 5-ALA is proposed as a theranostic agent in dentistry.
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