Related Experiment Video
Updated: Jan 30, 2026
A "Patient-Like" Orthotopic Syngeneic Mouse Model of Hepatocellular Carcinoma Metastasis
Published on: October 24, 2015
The expression profile of Jagged1 and Delta-like 4 in hepatocellular carcinoma
Areerat Kunanopparat1, Nattiya Hirankarn1, Jiraphorn Issara-Amphorn1
1Center of Excellence in Immunology and Immune Mediated Diseases, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Background:
Notch signaling has been linked to many cancers. However, there is still limited information about the expression and role of the Notch ligand in hepatocellular carcinoma (HCC).
Objective:
To investigate the expression of JAG1 and DLL4 in HCC tissues.
Methods:
One hundred and forty-five HCC tissues in paraffin block since 2009 to 2016 at King Chulalongkorn Memorial hospital were assayed for JAG1 and DLL4 by immunohistochemistry. All the sections were separately analyzed in tumor and adjacent non-tumor tissue and scoring based on intensity and quantity of immunoreaction. Kruskal-Wallis H test examined the correlation between JAG1 and DLL4 protein expression and clinical pathology.
Results:
The expression of JAG1 and DLL4 of tumor cells is 57.2% (83/145) and 88.9% (129/145), respectively. The expression of JAG1 is significantly higher in tumor tissues than adjacent non-tumor tissues (P = 0.002), and significantly increased in patients with age < 60 years old (P = 0.007). Interestingly, the DLL4 expression is also expressed in the normal liver tissue and DLL4 expression is not associated with any of the clinical parameters. When we performed a subgroup analysis, in HCC patients without a viral infection analysis, JAG1 is significantly increased in HCC patients with low albumin level (≤ 3.5) (P = 0.043).
Conclusions:
JAG1 expression is increased in HCC and seems to correlate with HCC patients with earlier onset and lower albumin level, whereas DLL4 expression did not significantly correlate with any clinical features.
Related Concept Videos
01:17The Delta-to-Delta Circuit
01:19The Y-to-Delta Circuit
The initial step in analyzing a wye-to-delta circuit is to assume a positive phase sequence. These phase voltages are then utilized to calculate the line voltages that occur directly across the delta-connected load impedances. Van, Vbn, and Vcn are the phase voltages in wye, and Vab, Vbc, and Vca are the line voltages for a delta circuit. The relation between...
01:16The Delta-to-Y Circuit
02:35Chromatin Position Affects Gene Expression
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
02:24Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
01:23Dipeptidyl Peptidase 4 Inhibitors