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Melosuavine I, an apoptosis-inducing bisindole alkaloid from Melodinus suaveolens
Zhong-Ying Fang1, Yi-Dan Ren2, Si-Yu Du3
1School of Biological Sciences and Technology, University of Jinan, Jinan 250022, PR China; Key laboratory of Natural Pharmaceutical Chemistry, Shandong University of Traditional Chinese Medicine, Jinan 250200, China.
Abstract:
A new bisindole alkaloid, melosuavine I (1) possessing an aspidosperma-aspidosperma dimeric skeleton, was isolated from the leaves of Melodinus suaveolens. The structure with absolute configuration of 1 was elucidated by a combination of MS, NMR and computational methods. MTT assays indicated that 1 exhibited significant cytotoxicity on human breast cancer BT549 cells with an IC50 value of 0.89 μM. Further study showed that 1 inhibited BT549 cell proliferation by inducing apoptosis through activation of caspase 3 and p53, and down-regulation of Bcl-2.
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