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Effects of intratracheal Fusarium solani inoculation in immunocompetent mice

Maiara Ignacio Costa1, Franciele Abigail Vilugron Rodrigues1, Flávia Franco Veiga1

  • 1Department of Clinical Analysis and Biomedicine, Laboratory of Medical Mycology, State University of Maringá, Brazil, Avenida. Colombo, 5790, CEP: 87020-900, Maringá, Paraná, Brazil.

Microbial Pathogenesis
|January 21, 2019
PubMed

Insights

Fusarium solani causes lethal lung damage in mice at high fungal loads. This study establishes a new intratracheal inoculation model for studying fusariosis in the respiratory tract.

Area of Science:

  • Mycology
  • Infectious Diseases
  • Immunology

Background:

  • Fusariosis, caused by Fusarium fungi, is an emerging infection, particularly in immunocompromised individuals.
  • Increasing incidence in immunocompetent hosts and limited understanding of host-pathogen interactions highlight research gaps.
  • Lack of standardized mammalian infection models, especially for respiratory tract fungal infections, impedes research.

Purpose of the Study:

  • To establish and validate an intratracheal inoculation model for Fusarium solani in immunocompetent mice.
  • To investigate the early host-pathogen interactions within the first 24 hours post-infection.
  • To assess the pathological consequences of F. solani infection in the murine respiratory tract.

Main Methods:

  • Intratracheal inoculation of F. solani microconidia into immunocompetent mice.
  • Assessment of fungal burden through Colony-Forming Unit (CFU) counts.
  • Histopathological analysis of lung tissues and examination of other organs for fungal dissemination.

Main Results:

  • High fungal burden of F. solani led to lethal pulmonary tissue damage.
  • Low fungal burden resulted in non-lethal pulmonary alterations and fungal presence in lungs.
  • No evidence of fungal dissemination to kidneys, spleen, liver, or heart within 24 hours.

Conclusions:

  • The intratracheal inoculation model effectively establishes Fusarium infection in the murine respiratory tract.
  • This model is suitable for studying Fusarium spp. pathogenesis and host-pathogen interactions.
  • Early pulmonary pathology and fungal burden are key factors in F. solani-induced disease severity.
Keywords:
FusariosisFusarium solaniMurine modelintratracheal instillation

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