Optical aptasensor based on silver nanoparticles for the colorimetric detection of adenosine

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Nanotechnology

Background:

• Adenosine (AD) plays a crucial role in various physiological processes.
• Accurate quantification of AD in biological fluids is essential for disease diagnosis.
• Existing methods for AD detection may lack sensitivity, selectivity, or simplicity.

Purpose of the Study:

• To develop a straightforward optical sensor for colorimetric determination of adenosine in human urine.
• To utilize silver nanoparticles (AgNPs) and anti-AD aptamer (AP) for sensitive and selective AD detection.
• To validate the sensor's performance in real human urine samples.

Main Methods:

• Development of a colorimetric sensor using AgNPs and anti-AD aptamer.
• Exploitation of AD-AP binding to induce dispersion of AgNPs and alter solution color.
• Monitoring absorbance changes for AD quantification.
• Comparison with a gold nanoparticle (AuNP)-based aptasensor.

Main Results:

• The sensor demonstrated a linear range of 60-280 nM for AD determination with a detection limit of 21 nM.
• High selectivity towards AD was observed, with minimal interference from other compounds.
• The method exhibited good precision, with relative standard deviations ranging from 4.8% to 8.8%.
• Successful application in detecting AD in human urine samples from lung cancer patients with high recovery rates (98-107%).

Conclusions:

• A novel and efficient optical sensor for adenosine detection in human urine has been successfully developed.
• The AgNPs-based aptasensor offers a sensitive, selective, and reliable method for AD quantification.
• This sensor holds promise for clinical diagnostics and biomedical research.