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Related Concept Videos

Crossing Over01:34

Crossing Over

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Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
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Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I,...
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Mate choice—the decision about whom to mate with—is a type of natural selection, since animals must reproduce to pass down their genes. Mate choice is also called intersexual selection because the behavior occurs between the sexes.
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Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
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Related Experiment Video

Updated: Jan 30, 2026

Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans
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Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans

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Cryptococcus neoformans Mating and Genetic Crosses.

Sheng Sun1, Shelby J Priest1, Joseph Heitman1

  • 1Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina.

Current Protocols in Microbiology
|January 21, 2019
PubMed
Summary
This summary is machine-generated.

This study details methods for genetic crosses in Cryptococcus fungal pathogens. Researchers can now observe mating and recover progeny for analyzing virulence and developing new antifungal strategies.

Keywords:
Cryptococcusgenetic crossmating progeny

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Area of Science:

  • Medical Mycology
  • Molecular Biology
  • Genetics

Background:

  • The Cryptococcus pathogenic species complex causes life-threatening cryptococcal meningoencephalitis.
  • High mortality rates underscore the need for effective treatments and preventative strategies.
  • Cryptococcus species are vital model organisms for studying fungal pathogenesis and genetics.

Purpose of the Study:

  • To provide a standardized protocol for performing genetic crosses in Cryptococcus.
  • To enable observation of mating phenotypes and meiotic processes.
  • To facilitate the recovery and analysis of meiotic progeny with novel traits.

Main Methods:

  • Detailed procedures for setting up genetic crosses between different Cryptococcus strains.
  • Techniques for observing and characterizing mating phenotypes.
  • Methods for isolating and collecting meiotic progeny for subsequent genetic and phenotypic analysis.

Main Results:

  • Successful establishment of genetic crosses in Cryptococcus.
  • Demonstration of observable mating phenotypes and meiotic structures.
  • Recovery of viable meiotic progeny for further research.

Conclusions:

  • The described methods provide a robust framework for Cryptococcus genetic research.
  • This protocol will aid in understanding fungal sexual reproduction and virulence.
  • Facilitates the generation of novel Cryptococcus strains for drug discovery and pathogenesis studies.