Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion01:27

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion

2.4K
The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are...
2.4K
Morphogenesis02:19

Morphogenesis

30.4K
Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.
30.4K
Coordination Number and Geometry02:57

Coordination Number and Geometry

19.0K
For transition metal complexes, the coordination number determines the geometry around the central metal ion. Table 1 compares coordination numbers to molecular geometry. The most common structures of the complexes in coordination compounds are octahedral, tetrahedral, and square planar.
19.0K
Coordination Compounds and Nomenclature02:54

Coordination Compounds and Nomenclature

26.7K
In most main group element compounds, the valence electrons of the isolated atoms combine to form chemical bonds that satisfy the octet rule. For instance, the four valence electrons of carbon overlap with electrons from four hydrogen atoms to form CH4. The one valence electron leaves sodium and adds to the seven valence electrons of chlorine to form the ionic formula unit NaCl (Figure 1a). Transition metals do not normally bond in this fashion. They primarily form coordinate covalent bonds, a...
26.7K
Lattice Centering and Coordination Number02:33

Lattice Centering and Coordination Number

11.5K
The structure of a crystalline solid, whether a metal or not, is best described by considering its simplest repeating unit, which is referred to as its unit cell. The unit cell consists of lattice points that represent the locations of atoms or ions. The entire structure then consists of this unit cell repeating in three dimensions. The three different types of unit cells present in the cubic lattice are illustrated in Figure 1.
Types of Unit Cells
Imagine taking a large number of identical...
11.5K
Structural Isomerism02:34

Structural Isomerism

21.7K
Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can...
21.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DHA-Rich Algae Feed Modulates Atlantic Salmon Health, Microbiota and Stress Response.

Aquaculture nutrition·2026
Same author

Replacing fish oil with Tetraselmis chui microalgae biomass does not compromise rainbow trout health: Biochemical, histologic, antioxidant and immune gene expression.

Scientific reports·2026
Same author

Embryo-scale Visual Cell Sorting reveals a conserved transcriptomic signature of nucleolar size linked to proteostasis.

bioRxiv : the preprint server for biology·2026
Same author

Development of a Human 3D Immune-Competent Neurovascular Model Enabling Time-Resolved Monitoring of Neuroinflammatory Dynamics and Neuroimmune Interactions.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Evolutionary transfer learning enables organism-wide inference of mammalian enhancer landscapes.

bioRxiv : the preprint server for biology·2026
Same author

T-cell receptors that are <i>k</i>-binding have defined sequence features.

Frontiers in immunology·2025

Related Experiment Video

Updated: Jan 30, 2026

Computational Reconstruction of Pancreatic Islets as a Tool for Structural and Functional Analysis
07:58

Computational Reconstruction of Pancreatic Islets as a Tool for Structural and Functional Analysis

Published on: March 9, 2022

1.9K

A Peninsular Structure Coordinates Asynchronous Differentiation with Morphogenesis to Generate Pancreatic Islets.

Nadav Sharon1, Raghav Chawla2, Jonas Mueller3

  • 1Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA.

Cell
|January 22, 2019
PubMed
Summary

Researchers discovered that pancreatic islet morphology and endocrine cell differentiation are directly linked. This finding challenges existing beliefs and could advance in vitro islet generation for diabetes treatment.

Keywords:
developmentdifferentiationendocrinehESCshuman embryonic stem cellsislets of Langerhansmorphogenesispancreassingle cell sequencing

More Related Videos

Pancreatic Islet Embedding for Paraffin Sections
09:09

Pancreatic Islet Embedding for Paraffin Sections

Published on: June 29, 2018

13.6K
A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice
06:56

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice

Published on: August 30, 2019

32.7K

Related Experiment Videos

Last Updated: Jan 30, 2026

Computational Reconstruction of Pancreatic Islets as a Tool for Structural and Functional Analysis
07:58

Computational Reconstruction of Pancreatic Islets as a Tool for Structural and Functional Analysis

Published on: March 9, 2022

1.9K
Pancreatic Islet Embedding for Paraffin Sections
09:09

Pancreatic Islet Embedding for Paraffin Sections

Published on: June 29, 2018

13.6K
A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice
06:56

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice

Published on: August 30, 2019

32.7K

Area of Science:

  • Developmental biology
  • Endocrinology
  • Stem cell biology

Background:

  • Pancreatic islets of Langerhans are crucial for glucose homeostasis.
  • Loss of insulin-producing beta cells leads to diabetes; islet transplantation is a potential cure.
  • Understanding islet development is key for in vitro islet production.

Purpose of the Study:

  • To elucidate the process of pancreatic islet development and morphogenesis.
  • To investigate the relationship between endocrine differentiation and islet structure.
  • To explore methods for generating functional islets in vitro.

Main Methods:

  • Single-cell mRNA sequencing was employed to analyze pancreatic islet development.
  • Observation of progenitor cell migration and differentiation dynamics.
  • Induction of peninsula-like structures in differentiating human embryonic stem cells.

Main Results:

  • Contrary to dogma, islet morphology and endocrine differentiation are directly related.
  • Differentiating endocrine progenitors form cohesive, bud-like structures called 'peninsulas'.
  • Alpha cells form the outer layer, followed by beta cells, establishing a core-mantle architecture.

Conclusions:

  • A direct spatiotemporal relationship exists between endocrine differentiation and islet morphogenesis.
  • The formation of peninsulas precedes the development of mature islet architecture.
  • Successful induction of peninsula-like structures offers a pathway for in vitro whole islet generation.