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Updated: Jan 30, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Whole genome sequencing of a ST2594 MRSA strain causing non-mucosal preoperative colonization and low-grade
Leonardo Ribeiro Bastos1, Maria Clara Fernandes Martins2, Rodolpho Mattos Albano3
1Department of Microbiology, Immunology and Parasitology, Medical Science School, State University of Rio de Janeiro, Avenida 28 de Setembro, 87, fundos, 3º andar, Rio de Janeiro, RJ, CEP: 20551-030, Brazil.
Abstract:
We present a post-operative infection caused by a methicillin-resistant Staphylococcus aureus strain, previously isolated in the preoperative screening, in a patient submitted to femoral osteosynthesis, successfully treated with oral ciprofloxacin. The isolate exhibited in vitro resistance to ciprofloxacin, Staphylococcal Cassette Chromosome mec type IV, it was negative for the lukS-PV Panton-Valentine leucocidin gene and belonged to ST2594 in multilocus sequence typing analysis. Whole genome sequencing revealed a genome size of 2,818,289 base pairs. The annotated genomes of ST2594 and N315 strains were compared, looking for genes related to virulence and resistance. The lack of the tst, sec, sel genes, associated with a mutation in the clfA gene, may partially explain the low morbity in this case.
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