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Nanoscale Metal-Organic Framework Based Two-Photon Sensing Platform for Bioimaging in Live Tissue
Chan Yang1, Kun Chen1, Mei Chen2
1Molecular Science and Biomedicine Laboratory, College of Chemistry and Chemical Engineering and College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, Collaborative Innovation Center for Chemistry and Molecular Medicine , Hunan University , Changsha 410082 , People's Republic of China.
Analytical Chemistry
|January 22, 2019
Summary
Researchers developed novel two-photon metal-organic framework (TP-MOF) probes for highly efficient biomedical sensing. These probes offer improved photostability and deep tissue imaging, overcoming limitations of existing nanoscale metal-organic frameworks (NMOFs).
Area of Science:
- Materials Science
- Nanotechnology
- Biomedical Engineering
Background:
- Nanoscale metal-organic frameworks (NMOFs) show promise for biomedical sensing.
- Existing NMOF probes face challenges in signal-to-noise ratio, photostability, and tissue penetration for biological systems.
- Developing efficient fluorescent probes for deep tissue imaging remains a significant hurdle.
Purpose of the Study:
- To introduce a novel two-photon metal-organic framework (TP-MOF) as an advanced sensing platform.
- To design and synthesize TP-MOFs incorporating target-responsive moieties for enhanced biomedical sensing.
- To demonstrate the capability of TP-MOF probes for sensing and imaging in biological samples.
Main Methods:
- Construction of TP-MOFs by incorporating target-responsive two-photon organic moieties into NMOFs via click chemistry.
- Covalent modification of PCN-58 (a model building block) with small-molecule probes for specific analytes (H₂S or Zn²⁺).
- Evaluation of TP-MOF probe properties including fluorescence response, photostability, selectivity, biocompatibility, and imaging depth.
Main Results:
- TP-MOF probes successfully retained the fluorescence-responsive properties of their organic components.
- Probes exhibited excellent photostability, selectivity, and biocompatibility.
- Near-infrared (∼820 nm) excited TP-MOF probes enabled sensing and imaging in live cells and tissue slices with 130 μm penetration depth.
Conclusions:
- The developed TP-MOF platform offers a highly efficient solution for biomedical sensing, addressing key limitations of NMOFs.
- The molecular design facilitates sensitive detection and deep-tissue imaging of biological targets.
- This approach is extendable to other MOFs and sensing components for diverse analytes of interest.
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