Related Experiment Video
Updated: Jan 30, 2026

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
Published on: March 8, 2012
Advanced approach to activity evaluation for released-active forms of antibodies to interferon-gamma by enzyme-linked
Еlena S Don1,2, Sergey А Bobrovnik3, Gordon Sherriff4
1a Department of Molecular and Cellular Pathophysiology , Federal State Budgetary Scientific Institute of General Pathology and Pathophysiology , Moscow , Russia.
Abstract:
Selection of a suitable assay to measure the activity of drugs based on released-active forms (RA forms of Abs) is an important step during their investigation. In this study, ELISA was utilized to examine the effect of RA forms of Abs to interferon gamma on the interaction between monoclonal anti-interferon gamma antibodies and human interferon gamma. The data showed that such RA forms of Abs are able to modulate the antibody interaction with interferon gamma, and it was suggested that the observed influence of RA forms of Abs on 'antibody-antigen' interaction could be used to analyze the activity of this kind of drugs.
More Related Videos
Related Concept Videos
Enzyme-linked Receptors
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Enzymes and Activation Energy
Activation Energy
tRNA Activation
Covalently Linked Protein Regulators
These groups modify specific amino acids in a protein....
Co-activators and Co-repressors

