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Related Experiment Video

Updated: Jan 30, 2026

Novel Process for 3D Printing Decellularized Matrices

Novel Process for 3D Printing Decellularized Matrices

Published on: January 7, 2019

Novel Process for 3D Printing Decellularized Matrices.

Stacey M S Gruber¹, Paulomi Ghosh², Karl Wilhelm Mueller²

¹Department of Biomedical Engineering, University of Cincinnati.

Journal of Visualized Experiments : Jove

|January 22, 2019

Summary

This summary is machine-generated.

This study developed a novel 3D bioprinting method using polycaprolactone (PCL) and polylactic acid (PLA) microspheres to protect decellularized extracellular matrix (DM). This approach enables the creation of functional tissue scaffolds at lower temperatures.

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Published on: October 9, 2020

Area of Science:

Biomaterials Engineering
Tissue Engineering
Regenerative Medicine

Background:

3D bioprinting seeks to fabricate biologically active scaffolds for tissue and organ regeneration.
High printing temperatures of some bioresorbable polymers (e.g., polylactic acid, PLA) degrade sensitive biologics.
Polycaprolactone (PCL) offers a lower, more biologically compatible printing temperature (65 °C).

Purpose of the Study:

To investigate the feasibility of 3D bioprinting decellularized extracellular matrix (DM) encapsulated within polylactic acid (PLA) microspheres using a polycaprolactone (PCL) filament.
To assess if the DM retains its functional conformation when protected by a PLA barrier during PCL-based 3D printing.
To evaluate the potential of this method for osteochondral repair applications.

Main Methods:

Decellularization of porcine cartilage to obtain decellularized extracellular matrix (DM).
Encapsulation of DM within polylactic acid (PLA) microspheres.
Extrusion of PLA-DM microspheres with polycaprolactone (PCL) into a composite filament.
Fabrication of 3D constructs using fused deposition modeling (FDM).

Main Results:

Successfully developed a composite filament containing PLA-encapsulated DM within a PCL matrix.
Produced 3D constructs via fused deposition modeling at a lower, biologically compatible temperature.
Preliminary evaluation of surface features for constructs with and without DM.

Conclusions:

3D bioprinting of decellularized extracellular matrix (DM) encapsulated in polylactic acid (PLA) microspheres within a polycaprolactone (PCL) matrix is feasible.
This method offers a promising strategy to incorporate sensitive biologics into 3D printed scaffolds for tissue engineering.
Further studies are warranted to confirm the biological efficacy and functional outcomes for osteochondral repair.