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In vitro susceptibility of dematiaceous fungi to nine antifungal agents determined by two different methods

Hailin Zheng1,2, Yun He1, Siyue Kan1

  • 1Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing, Jiangsu, China.

Mycoses
|January 22, 2019
PubMed
Abstract

Insights

The Sensititre YeastOne® method offers an easier and more efficient way to test antifungal susceptibility for dematiaceous fungi compared to the CLSI M38-A2 method. Agreement was good for common antifungals.

Area of Science:

  • Medical Mycology
  • Antimicrobial Susceptibility Testing
  • Infectious Diseases

Background:

  • Rising incidence of infections caused by dematiaceous fungi.
  • Limited empirical support for oral antifungal treatments.
  • Need for standardized and efficient antifungal susceptibility testing.

Purpose of the Study:

  • Compare the Sensititre YeastOne® colorimetric method with the CLSI M38-A2 broth microdilution method.
  • Evaluate antifungal susceptibility testing for dematiaceous fungi.
  • Establish a more effective treatment regimen for dematiaceous infections.

Main Methods:

  • Utilized two antifungal susceptibility testing methods: Sensititre YeastOne® and CLSI M38-A2.
  • Tested nine antifungal agents against 67 dematiaceous fungal isolates.
  • Determined Minimum Inhibitory Concentrations (MICs) and Minimum Encapsulated Concentrations (MECs).

Main Results:

  • MICs for itraconazole, voriconazole, posaconazole, and amphotericin B were lower than fluconazole.
  • High essential agreement between YeastOne® and CLSI methods for triazoles, 5-flucytosine, and amphotericin B.
  • Divergence observed with echinocandins, potentially due to methodology differences.

Conclusions:

  • The Sensititre YeastOne® method is easier and more efficient for dematiaceous fungi.
  • Good agreement between YeastOne® and CLSI methods for most common antifungals.
  • YeastOne® method shows promise for routine clinical use in managing dematiaceous fungal infections.
Keywords:
YeastOne® methodantifungal susceptibilitydematiaceous fungi

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