Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

pH Scale02:41

pH Scale

79.7K
Hydronium and hydroxide ions are present both in pure water and in all aqueous solutions, and their concentrations are inversely proportional as determined by the ion product of water (Kw). The concentrations of these ions in a solution are often critical determinants of the solution’s properties and the chemical behaviors of its other solutes. Two different solutions can differ in their hydronium or hydroxide ion concentrations by a million, billion, or even trillion times. A common means of...
79.7K
Scaling01:26

Scaling

593
In designing and analyzing filters, resonant circuits, or circuit analysis at large, working with standard element values like 1 ohm, 1 henry, or 1 farad can be convenient before scaling these values to more realistic figures. This approach is widely utilized by not employing realistic element values in numerous examples and problems; it simplifies mastering circuit analysis through convenient component values. The complexity of calculations is thereby reduced, with the understanding that...
593
Dimensional Analysis03:40

Dimensional Analysis

64.2K
Dimensional analysis, also known as the factor label method, is a versatile approach for mathematical operations. The main principle behind this approach is: the units of quantities must be subjected to the same mathematical operations as their associated numbers. This method can be applied to computations ranging from simple unit conversions to more complex and multi-step calculations involving several different quantities and their units.
Conversion Factors and Dimensional Analysis
The unit...
64.2K
Dimensional Analysis01:27

Dimensional Analysis

665
Dimensional analysis is a valuable technique in fluid mechanics for simplifying complex problems by reducing them into dimensionless groups. These groups capture the essential relationships between the variables involved, allowing researchers and engineers to analyze fluid flow without dealing with each variable individually. This approach reduces the number of independent variables, allowing for easier analysis and better understanding of physical phenomena.
In fluid mechanics, dimensional...
665
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.7K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.7K
Construction of Root Locus01:15

Construction of Root Locus

415
The construction of a root locus involves several key steps to analyze and visualize the behavior of a system's poles with varying gain. The number of branches in the root locus equals the number of closed-loop poles and is symmetrical about the real axis.
For positive gain values, the root locus exists on the real axis to the left of an odd number of finite open-loop poles or zeros. The root locus starts at the open-loop poles and traces the paths of the closed-loop poles as the gain...
415

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chitosan coating effects on biological response to niobia-modified yttria-stabilized zirconia.

The journal of advanced prosthodontics·2026
Same author

In Vitro and In Vivo Evaluation of Yttria-Niobia-Stabilized Zirconia Sandblasted Surface at Bone-Implant Interface with 3-Dimensional Visualization via Advanced Hard Tissue Clearing.

Biomaterials research·2026
Same author

Pressed lithium disilicate glass ceramics: Microstructural characterization, in vitro and in silico nonlinear dynamic analysis for biaxial flexural strength.

Journal of prosthodontics : official journal of the American College of Prosthodontists·2026
Same author

3D digital data analysis to identify factors influencing debonding of CAD-CAM resin composite molar crowns: A 4-year clinical study.

Journal of prosthodontic research·2026
Same author

Predicting and optimizing viscosity of dental resin composites with Gaussian process regression and Bayesian optimization.

Dental materials : official publication of the Academy of Dental Materials·2026
Same author

Antibacterial, mechanical, and bonding properties of self-adhesive resin composites containing surface pre-reacted glass ionomer filler.

Dental materials journal·2026

Related Experiment Video

Updated: Jan 30, 2026

Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle
05:49

Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle

Published on: April 27, 2021

3.0K

Fabricating large-scale three-dimensional constructs with living cells by processing with syringe needles.

Jun-Ichi Sasaki1, Chihiro Katata1,2, Gabriela L Abe1

  • 1Department of Biomaterials Science, Osaka University Graduate School of Dentistry, Suita 565-0871, Japan.

Journal of Biomedical Materials Research. Part A
|January 22, 2019
PubMed
Summary

Researchers developed a simple method to shape 3D cell constructs and remove dead cells using syringe needles. This technique improves cell viability and is promising for tissue engineering applications like nerve and blood vessel regeneration.

Keywords:
3D-shapingcell constructcell sortingfibroblastssyringe needle

More Related Videos

Automated Counterflow Centrifugal System for Small-Scale Cell Processing
04:49

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Published on: December 12, 2019

9.7K
Fabrication of Myogenic Engineered Tissue Constructs
13:43

Fabrication of Myogenic Engineered Tissue Constructs

Published on: May 1, 2009

10.9K

Related Experiment Videos

Last Updated: Jan 30, 2026

Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle
05:49

Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle

Published on: April 27, 2021

3.0K
Automated Counterflow Centrifugal System for Small-Scale Cell Processing
04:49

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Published on: December 12, 2019

9.7K
Fabrication of Myogenic Engineered Tissue Constructs
13:43

Fabrication of Myogenic Engineered Tissue Constructs

Published on: May 1, 2009

10.9K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Three-dimensional (3D) cell constructs are attractive biomaterials for tissue engineering.
  • Challenges include controlling construct morphology and eliminating dead cells postfabrication.
  • Moderate stress is hypothesized to shape constructs and remove dead cells.

Purpose of the Study:

  • To establish an easily available technology for shaping 3D cell constructs.
  • To eliminate dead cells postfabrication using mechanical stress.
  • To control the morphology of cell constructs.

Main Methods:

  • Spherical cell constructs of L-929 fibroblasts were fabricated.
  • Constructs were processed through syringe needles of varying sizes.
  • Cell viability was assessed using cell viability assays.

Main Results:

  • Large-scale rod-shaped cell constructs were successfully fabricated.
  • Construct diameter was controllable based on syringe needle size.
  • Cell viability in rod-shaped constructs exceeded 94%, indicating effective dead cell removal.

Conclusions:

  • Mechanical stress via syringe needle passage shapes 3D cell constructs and eliminates dead cells.
  • This technology offers a promising approach for fabricating elongated tissues.
  • Potential applications include the engineering of nerve and blood vessel tissues.