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MiR-744-3p regulates keratinocyte proliferation and differentiation via targeting KLLN in psoriasis

Changyuan Wang1,2, Jinbao Zong3, Yongxi Li2

  • 1Department of Dermatology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Experimental Dermatology
|January 22, 2019
PubMed

Insights

MicroRNA-744-3p is elevated in psoriasis and promotes keratinocyte proliferation while inhibiting differentiation. This microRNA targets KLLN, impacting psoriasis pathogenesis.

Area of Science:

  • Dermatology
  • Molecular Biology
  • Genetics

Background:

  • Psoriasis is a chronic inflammatory skin condition.
  • MicroRNAs (miRNAs) are implicated in psoriasis pathogenesis.
  • The specific role of miR-744-3p in psoriasis remains unclear.

Purpose of the Study:

  • To confirm the upregulation of miR-744-3p in psoriasis.
  • To investigate the function of miR-744-3p in keratinocyte proliferation and differentiation.
  • To identify and evaluate the role of miR-744-3p targets in psoriasis.

Main Methods:

  • Expression analysis of miR-744-3p in psoriatic samples.
  • In vitro studies on keratinocyte proliferation and differentiation.
  • Target prediction and validation for miR-744-3p, focusing on KLLN.

Main Results:

  • miR-744-3p was confirmed to be upregulated in psoriatic samples.
  • miR-744-3p significantly promoted keratinocyte proliferation and inhibited differentiation.
  • KLLN was identified as a direct target of miR-744-3p, with its expression reduced by miR-744-3p.
  • Overexpression of KLLN reversed the effects of miR-744-3p on keratinocytes.

Conclusions:

  • miR-744-3p is upregulated in psoriasis.
  • miR-744-3p regulates keratinocyte proliferation and differentiation by targeting KLLN.
  • This miR-744-3p/KLLN axis represents a potential therapeutic target for psoriasis.
Keywords:
KLLNdifferentiationkeratinocytesmiRNAproliferationpsoriasis

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