Related Experiment Video
Updated: Jan 30, 2026
A Model of Acute Lung Injury Following Visceral Ischemia-Reperfusion by Supra-Coeliac Aortic Cross Clamping in Rats
Published on: August 15, 2025
Topiramate Reduces Aortic Cross-Clamping-Induced Lung Injury in Male Rats
Aysel Kurt1, Yildiray Kalkan2, Hasan Turut3
1Private Practice, Department of Thoracic Surgery, Rize, Turkey.
Background:
Topiramate (TPM) decreases cytokine release and generation of reactive oxygen species (ROS). Cytokine and endothelin-1 (ET-1) secretion and ROS formation play an important role in ischemia-reperfusion (I/R) injury. We aimed to evaluate whether TPM prevents damage occurring in lung tissue during I/R.
Materials And Methods:
A total of 27 Wistar albino rats were divided into three groups of nine. To the I/R group, two hours of ischemia via infrarenal abdominal aorta cross-ligation and then two hours of reperfusion process were applied. TPM (100 mg/kg/day) orally for seven days was administered in the TPM treatment group. After the last dose of TPM treatment, respectively, two hours of ischemia and two hours of reperfusion were applied in this group.
Results:
Tumor necrosis factor-alpha (TNF-α) (p < 0.05), malondialdehyde (MDA) (p < 0.05), myeloperoxidase (MPO) (p < 0.05) and ET-1 (p < 0.05) levels of TPM treatment group's lung tissue were significantly lower than for the I/R group. Caspase-3 and histopathological damage were rather lower than that of the I/R group.
Conclusions:
During I/R, lung damage occurs due to excessive TNF-α and ET-1 release and ROS generation. TPM could well reduce development of lung damage by decreasing cytokine and ET-1 release and levels of ROS produced.
Insights
Topiramate (TPM) reduces lung damage from ischemia-reperfusion (I/R) injury. This study shows TPM decreases key inflammatory markers and oxidative stress, protecting lung tissue during I/R.
Area of Science:
- Pharmacology
- Pathophysiology
- Surgical Research
Background:
- Ischemia-reperfusion (I/R) injury involves cytokine and reactive oxygen species (ROS) release.
- Endothelin-1 (ET-1) also contributes to I/R-induced damage.
- Topiramate (TPM) is known to reduce cytokine release and ROS generation.
Purpose of the Study:
- To investigate the protective effects of TPM against lung injury during I/R.
- To evaluate TPM's impact on inflammatory markers and oxidative stress in lung tissue.
Main Methods:
- Wistar albino rats underwent 2 hours of ischemia followed by 2 hours of reperfusion.
- One group received TPM (100 mg/kg/day) orally for seven days prior to I/R.
- Lung tissue was analyzed for inflammatory mediators and oxidative stress markers.
Main Results:
- TPM treatment significantly reduced levels of tumor necrosis factor-alpha (TNF-α), malondialdehyde (MDA), myeloperoxidase (MPO), and ET-1 in lung tissue.
- Histopathological damage and caspase-3 levels were also lower in the TPM-treated group compared to the I/R group.
Conclusions:
- Lung damage during I/R is mediated by excessive TNF-α, ET-1, and ROS.
- TPM demonstrates potential in mitigating lung I/R injury by reducing these harmful mediators and oxidative stress.
Related Concept Videos
01:34Crossing Over
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process...
01:30Crossing Over
01:23Infertility in Males
01:20Monohybrid Crosses
01:50Cross-Sectional Research
01:18Dihybrid Crosses