Related Experiment Video

Updated: Jan 30, 2026

Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima
09:13

Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima

Published on: August 12, 2018

15.7K

p53/BNIP3-dependent mitophagy limits glycolytic shift in radioresistant cancer

Hyo Won Chang1, Mi Ra Kim2, Hyang Ju Lee3

  • 1Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.

Oncogene
|January 22, 2019
PubMed

Insights

This study reveals that p53 protein prevents cancer cells from shifting to glycolysis after radiation. Targeting glycolysis may overcome radioresistant cancers, with p53 status guiding patient selection.

Area of Science:

  • Oncology
  • Cancer Metabolism
  • Molecular Biology

Background:

  • The role of p53 in metabolic shifts induced by genotoxic cancer therapies remains unclear.
  • Understanding these shifts is crucial for developing effective cancer treatments.

Purpose of the Study:

  • To investigate the function of p53 in the metabolic reprogramming of head and neck squamous cell carcinoma cells post-irradiation.
  • To explore the link between p53, mitophagy, and metabolic adaptation in radioresistant cancer cells.

Main Methods:

  • Established isogenic p53-null and wild-type radioresistant head and neck squamous cell carcinoma cell lines.
  • Assessed cellular respiration, glycolysis, mitochondrial integrity, and mitophagy.
  • Investigated the role of BNIP3 in p53-mediated mitophagy.

Main Results:

  • p53-null cells exhibited decreased oxygen consumption, increased glycolysis, and mitochondrial dysfunction, enhancing sensitivity to glycolysis inhibitors.
  • Wild-type p53 cells maintained metabolic profiles and mitochondrial integrity via mitophagy.
  • A novel p53-mitophagy pathway (BNIP3-dependent) was identified, limiting the glycolytic shift by clearing damaged mitochondria.

Conclusions:

  • p53 plays a critical role in preventing radioresistant cancer cells from adopting a glycolytic phenotype.
  • Targeting glycolysis presents a potential strategy to treat radioresistant cancers.
  • p53 status can serve as a biomarker for patient stratification in clinical trials for glycolysis-targeting drugs.

More Related Videos

Yeast As a Chassis for Developing Functional Assays to Study Human P5314:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

10.0K
Author Spotlight: Assessment of Mitophagy Flux in Pancreatic β-Cells Using Effective and Robust Complementary Approaches07:04

Author Spotlight: Assessment of Mitophagy Flux in Pancreatic β-Cells Using Effective and Robust Complementary Approaches

Published on: September 15, 2023

2.2K

Related Concept Videos

Other Glycolytic Pathways01:24

Other Glycolytic Pathways

The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
903
Limiting Reactant02:27

Limiting Reactant

The relative amounts of reactants and products represented in a balanced chemical equation are often referred to as stoichiometric amounts. However, in reality, the reactants are not always present in the stoichiometric amounts indicated by the balanced equation.
70.0K
The Number e as a Limit01:29

The Number e as a Limit

The number e is a fundamental constant in calculus, playing a central role in describing continuous change, particularly exponential growth. It is most naturally defined through its relationship with the natural logarithm, which is the inverse of the exponential function with base e. This relationship allows e to be characterized using basic principles of differentiation rather than as an arbitrary numerical constant.A key property of the natural logarithm function, ln x, is that its derivative...
85
Types of Limits I01:23

Types of Limits I

Limits are a key mathematical concept for understanding how functions behave as their input approaches specific values, particularly when the function is undefined. They help reveal trends and discontinuities by examining the values a function approaches rather than its actual value.One-sided limits focus on the direction from which a value is approached. When a function behaves differently depending on whether the input approaches from the left or the right, the two one-sided limits may not...
179
Limit Laws I01:25

Limit Laws I

Limit laws provide essential tools for analyzing how functions behave as their input approaches a specific value. These laws are particularly useful when dealing with combinations of functions, provided the individual limits exist. The Sum and Difference Laws state that the limit of the sum or difference of two functions equals the sum or difference of their respective limits:The Product Law asserts that the limit of the product of two functions equals the product of their individual limits:A...
220
Introduction to Limits01:30

Introduction to Limits

A limit describes the value a function approaches as its input moves closer to a particular point. Even when a function is undefined at a specific value, limits allow us to analyze its behavior near that point. This concept is fundamental in calculus and essential for understanding continuity, derivatives, and integrals.Mathematically, a function f(x) has a limit L at x = a if its values L approach x as x gets arbitrarily close to a. This is written as:This notation expresses that the function...
229