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Chromosome fragmentation by chromosome mediated gene transfer
P N Goodfellow1, C A Pritchard
1Human Molecular Genetics Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London.
Cancer Surveys
|January 1, 1988
Summary
Chromosome mediated gene transfer (CMGT) creates human-mouse cell hybrids with human chromosome fragments. While not ideal for genetic mapping, CMGT effectively enriches specific human genome regions in somatic cell hybrids.
Area of Science:
- Genetics
- Molecular Biology
- Cell Biology
Background:
- Chromosome mediated gene transfer (CMGT) is a method for creating somatic cell hybrids containing human chromosome fragments.
- Human-X and human-Y chromosome panels were constructed using CMGT for further study.
Purpose of the Study:
- To construct and characterize human-mouse somatic cell hybrids containing fragments of the human X and Y chromosomes.
- To evaluate the utility of CMGT for genetic mapping and genome region enrichment.
Main Methods:
- Construction of two hybrid cell panels via CMGT: one with X chromosome fragments, another with Y chromosome fragments.
- Facilitation of Y chromosome panel construction using cotransfer with a selectable plasmid and antibody-dependent enrichment with monoclonal antibody 12E7.
- Detailed investigation of transferred fragment rearrangements and sequence retention.
Main Results:
- Extensive rearrangements were observed in the transferred chromosome fragments.
- Preferential retention of sequences from the centromeric region of the donor chromosomes was noted.
- CMGT proved effective for enriching specific human genome regions.
Conclusions:
- CMGT is not optimal for constructing detailed genetic maps due to fragment rearrangements.
- CMGT is a valuable technique for enriching specific human genome segments within somatic cell hybrids.