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A genetically encoded near-infrared fluorescent calcium ion indicator
Yong Qian1, Kiryl D Piatkevich2, Benedict Mc Larney3,4
1Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
Abstract:
We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca2+) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca2+ transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca2+ imaging in combination with other optogenetic indicators and actuators.
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