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Related Concept Videos

Peptide Bonds02:43

Peptide Bonds

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A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Targeted Cancer Therapies02:57

Targeted Cancer Therapies

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The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
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Methods Of Healthcare Delivery System01:26

Methods Of Healthcare Delivery System

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At the different levels of the healthcare system, we see varying methods of healthcare used. These methods include managed care systems, case management, and primary healthcare.
Managed Care System:
The managed care system is designed to control the cost while maintaining the quality of care. The patient's care from admission to discharge is planned by the primary care provider or the case manager, also known as the gatekeeper. In a managed care system, the number of care providers is...
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Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

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Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
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Drug Delivery: Overview01:16

Drug Delivery: Overview

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The selection of a drug's delivery route depends upon its physicochemical properties, including lipid or water solubility and ionization, as well as the therapeutic requirement, such as immediate or sustained effect. These routes can be divided into three primary categories: enteral, parenteral, and topical.
Enteral delivery involves administering drugs directly through swallowing, sublingual placement, or buccal application. Orally administered drugs predominantly navigate the...
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Updated: Jan 30, 2026

Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library
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Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library

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Syndiotactic peptides for targeted delivery.

Gaurav Jerath1, Ruchika Goyal1, Vishal Trivedi2

  • 1Molecular Informatics and Design Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.

Acta Biomaterialia
|January 22, 2019
PubMed
Summary
This summary is machine-generated.

New de novo designed peptides with alternating L- and D-amino acids show enhanced cellular uptake and stability in serum. These peptide-drug conjugates demonstrate selectivity for cancer cells, overcoming key challenges in targeted drug delivery.

Keywords:
Cancer therapyCell penetrating peptidesDrug delivery vehiclesProtein designTumor homing

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Area of Science:

  • Biomaterials Science
  • Drug Delivery Systems
  • Peptide Design

Background:

  • Current chemotherapy faces limitations due to lack of cell-type specificity and significant side effects.
  • Peptide-based biomaterials for targeted drug delivery are hindered by proteolytic susceptibility and poor specificity.
  • Existing peptide backbones, like poly-L, offer adaptability but increase vulnerability to degradation.

Purpose of the Study:

  • To design novel syndiotactic peptides with alternating L- and D-amino acids to overcome limitations of traditional peptide biomaterials.
  • To evaluate the cellular uptake, stability, and cell-type specificity of these de novo designed peptides.
  • To assess the therapeutic potential of peptide-drug conjugates for targeted cancer therapy.

Main Methods:

  • De novo design of syndiotactic peptides incorporating alternating L- and D-amino acids.
  • In vitro assessment of cellular uptake in breast and cervical cancer cells compared to Tat (48-60) peptide.
  • Evaluation of peptide stability in human plasma and bovine serum.
  • Analysis of peptide-drug conjugate toxicity towards cancerous and non-cancerous cells.

Main Results:

  • Designed peptides exhibited significantly higher cellular uptake rates than the Tat peptide in cancer cells.
  • Cellular uptake was found to be independent of concentration, temperature, and clathrin-mediated endocytosis.
  • The designed peptides demonstrated remarkable stability in both human plasma and bovine serum.
  • Peptide-drug conjugates showed reduced toxicity towards non-cancerous cells compared to cancer cells.

Conclusions:

  • Syndiotactic peptides represent a promising advancement in overcoming proteolytic susceptibility and non-specificity in targeted drug delivery.
  • The designed peptides offer enhanced cellular transduction, serum stability, and cancer cell selectivity.
  • These findings pave the way for developing effective peptide-based targeted drug delivery vectors and biomaterial conjugates.