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Related Experiment Videos

Plasmid "curing" by some recently synthetized 4-quinolone compounds.

L Selan1, F Scazzocchio, B Oliva

  • 1Microbiology Institute, Pharmacy Faculty, La Sapienza University, Rome, Italy.

Chemioterapia : International Journal of the Mediterranean Society of Chemotherapy
|October 1, 1988
PubMed
Summary

Nalidixic acid and 4-quinolones effectively eliminated plasmids from E. coli. Pefloxacin showed the highest efficacy in plasmid elimination compared to other tested quinolones.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Plasmids, such as F'lac and R-plasmids, are extrachromosomal DNA elements in bacteria like E. coli.
  • These plasmids can confer antibiotic resistance and other traits, making their elimination a target for therapeutic strategies.
  • Quinolones are a class of synthetic antibacterial agents with known antimicrobial activity.

Purpose of the Study:

  • To investigate the efficacy of nalidixic acid and newly synthesized 4-quinolones in eliminating F'lac and R-plasmids from E. coli.
  • To compare the plasmid elimination activity of different 4-quinolone derivatives.

Main Methods:

  • Bacterial cultures of E. coli harboring F'lac and R-plasmids were treated with varying concentrations of quinolone compounds.
  • Minimum Inhibitory Concentration (MIC) was determined for each compound.

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  • Plasmid elimination was assessed by culturing treated bacteria and checking for the presence or absence of plasmids.

    • Main Results:

    • Nalidixic acid and two novel 4-quinolones demonstrated plasmid elimination activity at sub-inhibitory concentrations (1/2 and 1/4 MIC).
    • Two out of three tested plasmids were eliminated by all tested quinolone derivatives, albeit with varying frequencies.
    • Pefloxacin exhibited the highest plasmid elimination efficacy among the tested compounds, while nalidixic acid was the least effective.

    Conclusions:

    • Sub-inhibitory concentrations of certain 4-quinolones are effective in eliminating clinically relevant plasmids from E. coli.
    • Pefloxacin is a promising candidate for further investigation in plasmid curing strategies.
    • The frequency of plasmid elimination varies depending on the specific quinolone compound and the plasmid type.