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Related Experiment Video

Updated: Jan 30, 2026

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Published on: May 7, 2018

Complex Phase Behavior of GUVs Containing Different Sphingomyelins.

Daniel Balleza¹, Andrea Mescola¹, Nathaly Marín-Medina²

¹Istituto Nanoscienze CNR, S3, Modena, Italy.

Biophysical Journal

|January 23, 2019

Summary

This summary is machine-generated.

Investigating lipid mixtures reveals complex membrane phases. Sphingolipids and specific lipid structures create distinct bilayer regions, offering insights into cell membrane organization.

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Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

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Published on: May 7, 2018

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In vitro Reconstitution of Cytoskeletal Networks inside Phase Separated Giant Unilamellar Vesicles (GUVs)

In vitro Reconstitution of Cytoskeletal Networks inside Phase Separated Giant Unilamellar Vesicles (GUVs)

Published on: June 20, 2025

Area of Science:

Membrane biophysics
Lipid bilayer organization
Cellular physiology

Background:

Biological membranes are crucial for cell function, with their lateral organization influencing physiological processes.
Ternary lipid bilayers (high/low melting lipids, cholesterol) model complex mammalian membranes.
Understanding lipid phase behavior is key to deciphering membrane functions.

Purpose of the Study:

To investigate the three-phase coexistence in model lipid bilayers.
To explore the role of sphingomyelin (SM) and diphytanoyl-phosphatidylcholine in membrane phase behavior.
To understand the factors influencing the formation of multiple fluorescence intensity levels in lipid mixtures.

Main Methods:

Utilizing giant unilamellar vesicles (GUVs) for fluorescence microscopy.
Analyzing lipid mixtures containing diphytanoyl-phosphatidylcholine, cholesterol, and various sphingomyelins (SMs).
Observing and quantifying fluorescence intensity levels in lipid bilayers at different temperatures.

Main Results:

Specific lipid mixtures containing SMs and diphytanoyl-phosphatidylcholine exhibited more than two fluorescence intensity levels at low temperatures.
These multi-intensity levels are linked to the presence of long and asymmetric lipid chains.
Some liposomes in a three-phase coexistence state showed high sensitivity to lateral tension.

Conclusions:

The asymmetric structure of sphingolipids and interdigitation effects likely contribute to the observed multi-phase behavior.
This study provides insights into the complex lateral organization of biological membranes.
The findings highlight the importance of lipid composition in determining membrane phase behavior and potential sensitivity to mechanical forces.