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Updated: Jan 30, 2026

Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
Covalently Palladated Oligonucleotides Through Oxidative Addition of Pd0
Harri Räisälä1, Tuomas Lönnberg1
1Department of Chemistry, University of Turku, Vatselankatu 2, 20014, Turku, Finland.
Abstract:
An 11-mer oligonucleotide incorporating a central (2-iodobenzoylamino)methyl residue has been synthesized and palladated by oxidative addition of Pd2 (dba)3 . UV melting profiles of the duplexes formed by the palladated oligonucleotide with its natural complements were biphasic and the higher melting temperatures (Tm ) exhibited considerable hysteresis. CD spectra, in turn, resembled those of canonical B-type double helices. Two-step denaturation, with the "low-Tm " melting involving only canonical base pairs and the "high-Tm " melting involving also dissociation of a PdII -mediated base pair, appears the most likely explanation for the observed UV melting profiles. As the latter step in all cases takes place at a higher temperature than denaturation of natural duplexes of the same length, the putative PdII -mediated base pairs are stabilizing.
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