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Updated: Jan 30, 2026

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
Published on: September 5, 2013
Real-time PCR followed by high-resolution melting analysis - a new robust approach to evaluate SCCmec typing of
Yasaman Sadeghi1, Seyed Alireza Salami2, Pegah Kananizadeh1
1Department of Pathobiology, School of Public Health, and Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Aim:
We designed a novel approach based on real-time PCR followed by high-resolution melting (HRM) analysis to determine the Staphylococcal cassette chromosome mec (SCCmec) types of methicillin-resistant Staphylococcus aureus strains, which we compared against the results of conventional multiplex PCR SCCmec typing.
Methods:
Multiplex PCR (for ccr and mec gene complexes) was carried out as conventional method. The HRM analysis was then designed using standard strains of each SCCmec type.
Results:
The M-PCR results included types III (33.33%), IV (43.33%) and V (23.33%). HRM analysis was able to distinguish all five types, which were used to set up the protocol with a sensitivity and specificity of 100% compared with the conventional method.
Conclusion:
This novel method for SCCmec typing has high specificity and sensitivity and can be conducted in a shorter period of time at lower costs.
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